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GCNT3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6006418

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GCNT3, Polyclonal Antibody
Full Product Name
GCNT3, CT (GCNT3, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase)
Product Gene Name
anti-GCNT3 antibody
Product Synonym Names
Anti -GCNT3, CT (GCNT3, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
50,864 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (59903982..59912210). Location: 15q21.3
OMIM
606836
3D Structure
ModBase 3D Structure for O95395
Immunogen
GCNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-418 amino acids from the C-terminal region of human GCNT3.

Product Categories/Family for anti-GCNT3 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase
Applications Tested/Suitable for anti-GCNT3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-GCNT3 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-GCNT3 antibody

GCNT3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).
Rabbit Anti-GCNT3 Polyclonal Antibody – Cat# MBS6006418

Immunohistochemistry (IHC) of anti-GCNT3 antibody

GCNT3 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCNT3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-GCNT3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6006418

Flow Cytometry (FC/FACS) of anti-GCNT3 antibody

GCNT3 Antibody (C-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-GCNT3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6006418

Other Notes

Small volumes of anti-GCNT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCNT3 purchase

MBS6006418 is an antibody targeting GCNT3. Also known as GCNT3, CT (GCNT3, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GCNT3. It may not necessarily be applicable to this product.

NCBI GI #
16877562
NCBI GeneID
9245
NCBI Accession #
AAH17032.1
UniProt Primary Accession #
O95395
UniProt Related Accession #
O95395
NCBI Official Full Name
GCNT3 protein
NCBI Official Synonym Full Names
glucosaminyl (N-acetyl) transferase 3, mucin type
NCBI Official Symbol
GCNT3
NCBI Official Synonym Symbols
GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
UniProt Protein Name
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
UniProt Synonym Protein Names
C2GnT-mucin type; C2GnT-M; hC2GnT-M; Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
Protein Family
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
UniProt Gene Name
GCNT3
UniProt Synonym Gene Names
C2GnT-M; hC2GnT-M; C2/4GnT
UniProt Entry Name
GCNT3_HUMAN
NCBI Protein Information
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; hC2GnT-M; C2GnT-mucin type; beta1,6GlcNAc-transferase; beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

UniProt Comments for GCNT3

GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.

Protein type: EC 2.4.1.150; EC 2.4.1.102; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; tissue morphogenesis; cellular protein metabolic process; O-glycan processing; immunoglobulin production in mucosal tissue; carbohydrate metabolic process; post-translational protein modification; intestinal absorption
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Research Articles on GCNT3

1. Data show that the Golgi docking of vesicular complexes (VCs) use different golgins for docking: C2GnT-M-carrying VC (C2GnT-M-VC) utilizes Giantin, whereas C1GalT1-VC employs GM130-GRASP65 complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GCNT3 antibody

 Reference Product  PubMed Publications
 MUC6 antibody  >2 publications with GCNT3 and MUC6
 MUC2 antibody  >1 publications with GCNT3 and MUC2
 MUC5B antibody  >1 publications with GCNT3 and MUC5B

Pathways associated with anti-GCNT3 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mucin Type O-Glycan Biosynthesis Pathway antibodies  Mucin Type O-Glycan Biosynthesis Pathway Diagram
 Mucin Type O-Glycan Biosynthesis Pathway antibodies  Mucin Type O-Glycan Biosynthesis Pathway Diagram
 O-glycan Biosynthesis, Mucin Type Core Pathway antibodies  O-glycan Biosynthesis, Mucin Type Core Pathway Diagram
 O-glycan Biosynthesis, Mucin Type Core Pathway antibodies  O-glycan Biosynthesis, Mucin Type Core Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram

Diseases associated with anti-GCNT3 antibody

 Disease Name  Pubmed Publications
 Hyperplasia Antibodies  >2 publications with GCNT3 and Hyperplasia
 Lung Diseases Antibodies  >1 publications with GCNT3 and Lung Diseases
 Immune System Diseases Antibodies  >1 publications with GCNT3 and Immune System Diseases

Organs/Tissues associated with anti-GCNT3 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >2 publications with GCNT3 and Intestine
 Eye Antibodies  >1 publications with GCNT3 and Eye
 Lung Antibodies  >1 publications with GCNT3 and Lung
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