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DDX5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600652

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DDX5, Monoclonal Antibody
Full Product Name
DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68)
Product Gene Name
Product Synonym Names
Anti -DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C102
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human DDX5 at ~69kD. Species Crossreactivity: mouse, rat
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
75,619 Da
Chromosome Location
Chromosome: 19; NC_007317.4 (50218186..50226015, complement)
3D Structure
ModBase 3D Structure for Q3SYZ5
Immunogen
Partial sequence of recombinant full-length protein to human DDX5

Product Categories/Family for anti-DDX5 antibody

Applications Tested/Suitable for anti-DDX5 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-DDX5 antibody
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug/sample
Immunocytochemistry: 2-100ug/ml

Immunostaining (IS) of anti-DDX5 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS600652.
Mouse Anti-DDX5 Monoclonal Antibody – Cat# MBS600652

Western Blot (WB) of anti-DDX5 antibody

Detection of DDX5 by Western Blot. Samples: Whole cell lysate from human HeLa (25ug) , mouse NIH3T3 (25ug) and rat F2408 (25ug) cells. Predicted molecular weight: 69kD
Mouse Anti-DDX5 Monoclonal Antibody, Western Blot (WB) – Cat# MBS600652

Immunoprecipitation (IP) of anti-DDX5 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS600652.
Mouse Anti-DDX5 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS600652

Testing Data of anti-DDX5 antibody

Mouse Anti-DDX5 Monoclonal Antibody, Testing Data – Cat# MBS600652

Other Notes

Small volumes of anti-DDX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DDX5 purchase

MBS600652 is an antibody targeting DDX5. Also known as DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DDX5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 isoform 2
NCBI Official Symbol
DDX5
UniProt Protein Name
DDX5 protein
UniProt Gene Name
DDX5
UniProt Entry Name
Q3SYZ5_BOVIN
NCBI Protein Information
probable ATP-dependent RNA helicase DDX5

UniProt Comments for DDX5

Sequence similarities: Belongs to the DEAD box helicase family. RuleBase RU000492

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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