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Nestin (NES) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600695

Nestin, a large intermediate filament protein (class Type VI), is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Nestin, Monoclonal Antibody
Full Product Name
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Product Gene Name
anti-NES antibody
Product Synonym Names
Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q178
Host
Mouse
Species Reactivity
Mouse, Rat, Rodent
Form/Format
Supplied as a liquid in Tris-Glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.
Purity/Purification
Purified
Purified
Specificity
Recognizes rodent Nestin at 200-220kD. Species Crossreactivity: mouse and rat. Does not crossreact with human.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
177,439 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (156638555..156647189, complement). Location: 1q23.1
OMIM
600915
3D Structure
ModBase 3D Structure for P48681
Immunogen
Nestin purified from embryonic rat spinal cord.

Product Categories/Family for anti-NES antibody

Antibodies; Abs to Neurofilament
Applications Tested/Suitable for anti-NES antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-NES antibody
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.
Dilution: Immunohistochemistry (Frozen or paraffin): 1:20-1:200. Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.
Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70 degree C will show breakdown of nestin sometimes into multiple fragments.
Immunocytochemistry: 1:20-1:200. Fix cells in 4% paraformaldehyde buffered with 50mM sodium borate, pH 9.5.

Immunohistochemistry (IHC) of anti-NES antibody

Nestin staining of mouse pancreas using MBS600695. Tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.
Mouse Anti-NES Monoclonal Antibody – Cat# MBS600695

Immunohistochemistry (IHC) of anti-NES antibody

Negative control staining of mouse pancreas using MBS600695. Tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, using IHC-Select Detection with HRP-DAB. No signal detected.
Mouse Anti-NES Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS600695

Testing Data of anti-NES antibody

Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin using MBS600695. The blue is Nissl stain (rough ER), the red is nestin (MBS600695), and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes). Photo courtesy of Robert Wine, NIEHS.
Mouse Anti-NES Monoclonal Antibody, Testing Data – Cat# MBS600695

Other Notes

Small volumes of anti-NES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NES purchase

MBS600695 is an antibody targeting NES. Also known as Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes). Reactive in Mouse, Rat, Rodent. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NES. It may not necessarily be applicable to this product.

NCBI GI #
38176300
NCBI GeneID
10763
NCBI Accession #
NP_006608.1
UniProt Primary Accession #
P48681
UniProt Secondary Accession #
O00552; Q3LIF5; Q5SYZ6
UniProt Related Accession #
P48681; Q05BW3; Q2YDX4; Q9H6U9
GenBank Nucleotide #
NM_006617.1
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
FLJ21841; Nbla00170
UniProt Protein Name
Nestin
Protein Family
Nestin
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
NCBI Protein Information
nestin; OTTHUMP00000032198

NCBI Summary for NES

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
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UniProt Comments for NES

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; brain development; negative regulation of neuron apoptosis; cell projection morphogenesis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
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Research Articles on NES

1. Expression of embryonic stem cell markers SOX2 and nestin in dermatofibrosarcoma protuberans and dermatofibroma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NES antibody

 Reference Product  PubMed Publications
 CDK1 antibody  >3 publications with NES and CDK1

Diseases associated with anti-NES antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >178 publications with NES and Neoplasms
 Cardiovascular Diseases Antibodies  >61 publications with NES and Cardiovascular Diseases
 Vascular Diseases Antibodies  >45 publications with NES and Vascular Diseases
 Leukemia Antibodies  >42 publications with NES and Leukemia
 Congenital Abnormalities Antibodies  >36 publications with NES and Congenital Abnormalities
 Breast Neoplasms Antibodies  >36 publications with NES and Breast Neoplasms
 Neurodegenerative Diseases Antibodies  >29 publications with NES and Neurodegenerative Diseases
 Pain Antibodies  >29 publications with NES and Pain
 Lung Diseases Antibodies  >24 publications with NES and Lung Diseases
 Adenocarcinoma Antibodies  >20 publications with NES and Adenocarcinoma

Organs/Tissues associated with anti-NES antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >151 publications with NES and Brain
 Nerve Antibodies  >91 publications with NES and Nerve
 Muscle Antibodies  >59 publications with NES and Muscle
 Heart Antibodies  >51 publications with NES and Heart
 Eye Antibodies  >38 publications with NES and Eye
 Vascular Antibodies  >36 publications with NES and Vascular
 Kidney Antibodies  >34 publications with NES and Kidney
 Embryonic Tissue Antibodies  >33 publications with NES and Embryonic Tissue
 Lung Antibodies  >26 publications with NES and Lung
 Skin Antibodies  >24 publications with NES and Skin
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