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Cd60b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600804

Human CD60 antigen is a glycolipid determinant found on about 30% of peripheral blood T cells. The glycolipid determinant is linked to a glycoprotein which can transduce activation signals. More details

$830 / 120 Tests +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD60b, Monoclonal Antibody
Full Product Name
CD60b (FITC)
Product Synonym Names
Anti -CD60b (FITC)
Clonality
Monoclonal
Isotype
IgM
Clone Number
7H226
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Purity/Purification
Purified
Purified
Specificity
Recognizes human CD60b. Recognizes a 9-0-acetylated disialosyl group linked to the glycoprotein CD60. Will activiate T cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Immunogen
Human rheumatoid synovial T cell line (ST-1)

Product Categories/Family for anti-CD60b antibody

Antibodies; Abs to CD Markers
Applications Tested/Suitable for anti-CD60b antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CD60b antibody
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot.
Dilution: Flow Cytometry: 1:50; 80ul labels 5x10e5 ficoll prepared human PBL cells.
Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human PBL were incubated 45 minutes on ice with 80ul of C2413-79B at a 1:50 dilution.
2. Cells were then washed three times, fixed and analyzed by FACS. A 39% sub population of the cells stained positive with a mean shift of 2.3 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration.
3. Binding was blocked when cells were pre incubated for 10 minutes with 20ul of 0.5mg/ml anti-CD60b antibody (C2413-79).

Testing Data of anti-CD60b antibody

Binding of MBS600804 to ficoll prepared human PBL.
Mouse Anti-Cd60b Monoclonal Antibody – Cat# MBS600804

Other Notes

Small volumes of anti-CD60b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD60b purchase

MBS600804 is an antibody targeting CD60b. Also known as CD60b (FITC). Reactive in Human. Host: Mouse.
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