Mouse Anti-X-Prolyl Aminopeptidase Monoclonal Antibody – Cat# MBS600856

X-Prolyl Aminopeptidase Antibody – Mouse Monoclonal

Name: X-Prolyl Aminopeptidase Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS600856
Price: 1000 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600856

Membrane-bound Aminopeptidase P2 is a hydrolase specific for N-terminal imido bonds. It is widely expressed in many adult tissues with the highest levels in the kidney. More details

$1000 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

X-prolyl aminopeptidase (Xpnpep1), Monoclonal Antibody

Full Product Name

X-prolyl aminopeptidase (aminopeptidase P, 2 Aminoacylproline aminopeptidase, mAmP, Membrane-bound aminopeptidase P, Membrane-bound AmP, Membrane-bound APP, Xaa-Pro aminopeptidase 2 precursor, X-Pro aminopeptidase 2)

Product Gene Name

anti-Xpnpep1 antibody

Product Synonym Names

Anti -X-prolyl aminopeptidase (aminopeptidase P, 2 Aminoacylproline aminopeptidase, mAmP, Membrane-bound aminopeptidase P, Membrane-bound AmP, Membrane-bound APP, Xaa-Pro aminopeptidase 2 precursor, X-Pro aminopeptidase 2)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

8B54

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human X-prolyl aminopeptidase. Does not crossreact with recombinant human XPNEP-1 or recombinant mouse XNEP-2. Species Sequence Homology: chimpanzee; 95%, canine; 84%, rat; 82%, mouse; 81%.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

69,591 Da

Chromosome Location

chromosome: 19; Location: 19 D2; 19

3D Structure

ModBase 3D Structure for Q3UKF5

Immunogen

NS0-derived recombinant human X-prolyl aminopeptidase (aa23-650, Accession #O43895).

Product Categories/Family for anti-Xpnpep1 antibody

Antibodies; Abs to Enzymes

Applications Tested/Suitable for anti-Xpnpep1 antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization

Application Notes for anti-Xpnpep1 antibody

Suitable for use in Western Blot, Immunoprecipitation, ELISA and Neutralization.
Dilution: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human X-prolyl aminopeptidase from conditioned cell medium.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.
Neutralization: Inhibits/neutralizes the enzymatic activity of recombinant human X-prolyl aminopeptidase. X1049-56A was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with K(Abz)PP-pNA. 50% of the proteolytic activity was inhibited by X1049-56A at ~1.5ug/ml (IC50) under conditions in which the enzyme was present at 2ug/ml and the substrate concentration was 0.05mM. Considering the molecular masses of the enzyme (72kD) and the antibody (150kD), IC50 was achieved at ~1:2 molar ratio of the antibody to the enzyme.

Other Notes

Small volumes of anti-Xpnpep1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Xpnpep1 purchase

MBS600856 is an antibody targeting Xpnpep1. Also known as X-prolyl aminopeptidase (aminopeptidase P, 2 Aminoacylproline aminopeptidase, mAmP, Membrane-bound aminopeptidase P, Membrane-bound AmP, Membrane-bound APP, Xaa-Pro aminopeptidase 2 precursor, X-Pro aminopeptidase 2). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Xpnpep1. It may not necessarily be applicable to this product.

NCBI GI #

94962412

NCBI GeneID

170750

UniProt Primary Accession #

Q3UKF5

UniProt Related Accession #

Q3TL27; Q3UE92; Q3UKF5; Q6P1B1; Q8BKH1; Q8BV43; Q8VDK8

NCBI Official Full Name

X-prolyl aminopeptidase

NCBI Official Synonym Full Names

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble

NCBI Official Symbol

Xpnpep1

NCBI Official Synonym Symbols

D230045I08Rik; Xpnpep1

UniProt Protein Name

X-prolyl aminopeptidase

Protein Family

Probable proline iminopeptidase

UniProt Gene Name

Xpnpep1

UniProt Entry Name

Q3UKF5_MOUSE

NCBI Protein Information

xaa-Pro aminopeptidase 1; sAmp; X-Pro aminopeptidase 1; soluble aminopeptidase P; cytosolic aminopeptidase P; aminoacylproline aminopeptidase; X-prolyl aminopeptidase 1, soluble

UniProt Comments for Xpnpep1

XPNPEP1: Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Belongs to the peptidase M24B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.11.9; Protease

Cellular Component: cytoplasm; cytosol

Molecular Function: peptidase activity; protein homodimerization activity; hydrolase activity; metallopeptidase activity; manganese ion binding; metal ion binding; aminopeptidase activity

Biological Process: proteolysis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-Xpnpep1 antibody

Disease Name	Pubmed Publications
Hypertension Antibodies	>1 publications with Xpnpep1 and Hypertension
Myocardial Infarction Antibodies	>1 publications with Xpnpep1 and Myocardial Infarction
Fibrosis Antibodies	>1 publications with Xpnpep1 and Fibrosis
Congenital Abnormalities Antibodies	>1 publications with Xpnpep1 and Congenital Abnormalities
Kidney Diseases Antibodies	>1 publications with Xpnpep1 and Kidney Diseases

Organs/Tissues associated with anti-Xpnpep1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with Xpnpep1 and Blood
Liver Antibodies	>1 publications with Xpnpep1 and Liver
Heart Antibodies	>1 publications with Xpnpep1 and Heart
Kidney Antibodies	>1 publications with Xpnpep1 and Kidney
Brain Antibodies	>1 publications with Xpnpep1 and Brain