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Class 2, I-Ek (MHC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS600924

Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2 More details

$705 / 0.5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MHC Class 2, I-Ek, Monoclonal Antibody
Full Product Name
MHC Class 2, I-Ek (Azide Free)
Product Synonym Names
Anti -MHC Class 2, I-Ek (Azide Free)
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
14-4-4S
Host
Mouse
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS. No preservative added.
Purity/Purification
Purified
Purified
Specificity
Recognizes an epitope on mouse I-Ek MHC class II alloantigen. Reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Has been reported to crossreact with the rat class II alloantigen RT1D.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
C3H mouse skin graft and splenocytes

Product Categories/Family for anti-MHC Class 2, I-Ek antibody

Antibodies; Abs to MHC
Applications Tested/Suitable for anti-MHC Class 2, I-Ek antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-MHC Class 2, I-Ek antibody
Flow cytometry1, , Complement-dependent cytotoxicity1, , Immunoprecipitation3, , In vitro induction of antigen-specific B-cell differentiation4, , In vitro blocking of antigen presentation5, , Immunohistochemistry (acetone-fixed, frozen tissue sections)6

Other Notes

Small volumes of anti-MHC Class 2, I-Ek antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MHC Class 2, I-Ek purchase

MBS600924 is an antibody targeting MHC Class 2, I-Ek. Also known as MHC Class 2, I-Ek (Azide Free). Reactive in Mouse. Host: Mouse.
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