Rabbit Anti-ISWI Polyclonal Antibody – Cat# MBS6009337

ISWI Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).

ISWI Antibody – Rabbit Polyclonal

Name: ISWI Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6009337
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6009337

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ISWI, Polyclonal Antibody

Full Product Name

ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog)

Product Gene Name

anti-Iswi antibody

Product Synonym Names

Anti -ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

118,873 Da

Chromosome Location

Chromosome: 2R; NT_033778.3 (8524176..8528133). Location: 49B10-49B10

3D Structure

ModBase 3D Structure for Q24368

Immunogen

ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human ISWI.

Product Categories/Family for anti-Iswi antibody

Antibodies; Abs to Actin Related Proteins

Applications Tested/Suitable for anti-Iswi antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-Iswi antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-Iswi antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Iswi purchase

MBS6009337 is an antibody targeting Iswi. Also known as ISWI, NT (SMARCA5, SNF2H, WCRF135, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, Sucrose nonfermenting protein 2 homolog). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Iswi. It may not necessarily be applicable to this product.

NCBI GI #

24653134

NCBI GeneID

36390

NCBI Accession #

NP_725204.1

UniProt Primary Accession #

Q24368

UniProt Secondary Accession #

Q0E9A4; Q8SX14; Q9V6E8; A4UZF0; B5X0J3

UniProt Related Accession #

Q24368

GenBank Nucleotide #

NM_165931.2

NCBI Official Full Name

imitation SWI, isoform C

NCBI Official Synonym Full Names

Imitation SWI

NCBI Official Symbol

Iswi

NCBI Official Synonym Symbols

ACF; anon-EP1279744.124; CG8625; CHRAC; dCHRAC; dISWI; DmelCG8625; dNURF; ISW; iswi; ISWI; NURF; Nurf-140; NURF-140; p140; p140/ISWI; SWI

UniProt Protein Name

Chromatin-remodeling complex ATPase chain Iswi

UniProt Synonym Protein Names

CHRAC 140 kDa subunit; Nucleosome-remodeling factor 140 kDa subunit; NURF-140; Protein imitation swi

Protein Family

Chromatin-remodeling complex ATPase

UniProt Gene Name

Iswi

UniProt Synonym Gene Names

NURF-140

UniProt Entry Name

ISWI_DROME

NCBI Protein Information

CG8625 gene product from transcript CG8625-RA; CG8625-PA; CG8625-PB; CG8625-PC; ISWI ATPase; Iswi-PA; Iswi-PB; Iswi-PC; imitation SW; imitation SWI; imitation switch; imitation-SWI; nucleosome remodeling factor; nucleosome remodeling factor - 140kD

UniProt Comments for Iswi

Function: Energy-transducing component of the chromatin-remodeling complexes NURF (nucleosome-remodeling factor), ACF (ATP-utilizing chromatin assembly and remodeling factor), and CHRAC (chromatin accessibility complex). NURF catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. It is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis. Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure: Component of the NURF complex composed of Caf1, E(bx), Nurf-38 and Iswi. Ref.6

Subcellular location: Nucleus Ref.6.

Developmental stage: Present throughout embryonic, larval and pupal development and in female adults. Present at low levels in adult males (at protein level). Ref.6

Sequence similarities: Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 2 SANT domains.

Research Articles on Iswi

1. finding show all fundamental aspects of nucleosome-remodeling catalysis are contained within the compact ATPase domain of ISWI

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Iswi antibody

Reference Product	PubMed Publications
Acf1 antibody	>24 publications with Iswi and Acf1
Caf1 antibody	>6 publications with Iswi and Caf1
Nurf-38 antibody	>6 publications with Iswi and Nurf-38
Trf2 antibody	>3 publications with Iswi and Trf2
Chrac-14 antibody	>2 publications with Iswi and Chrac-14
Chrac-16 antibody	>2 publications with Iswi and Chrac-16

Organs/Tissues associated with anti-Iswi antibody

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>7 publications with Iswi and Embryonic Tissue
Ovary Antibodies	>6 publications with Iswi and Ovary
Testis Antibodies	>4 publications with Iswi and Testis
Head Antibodies	>3 publications with Iswi and Head