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Mast Cell Protease 11 (PRSS34) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601022

Mast Cell Protease 11 (MCP-11) encoded by the Prss34 gene is a secreted trypsin-like serine protease. It consists of a signal peptide (aa 1 to 19), a pro region (aa 20 to 34), and a catalytic domain (aa 35 to 318). The amino acid sequence of mouse MCP-11 is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mast Cell Protease 11 (Prss34), Monoclonal Antibody
Full Product Name
Mast Cell Protease 11 (MCP-11, Prss34)
Product Gene Name
Product Synonym Names
Anti -Mast Cell Protease 11 (MCP-11, Prss34)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8B33
Host
Rat
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.
Purity/Purification
Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse MCP-11/Prss34.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 17; NC_000083.6 (25298394..25300161). Location: 17 A3.3; 17 12.53 cM
Immunogen
NS0-derived recombinant mouse MCP-11/Prss34.

Product Categories/Family for anti-Prss34 antibody

Applications Tested/Suitable for anti-Prss34 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-Prss34 antibody
Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Dilution: Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MCP-11. The detection limit for rmMCP-11 is approximately 10 ng/well.
Western Blot: 1-2ug/ml with the appropriate secondary reagents to detect mouse MCP-11. Using a colorimetric detection system, the detection limit for rmMCP-11 is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml to immunoprecipitate rmMCP-11 from conditioned cell culture medium. The recovered rmMCP-11 can be detected in Western blots using a polyclonal antibody.

Other Notes

Small volumes of anti-Prss34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Prss34 purchase

MBS601022 is an antibody targeting Prss34. Also known as Mast Cell Protease 11 (MCP-11, Prss34). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Prss34. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
mastin
NCBI Official Synonym Full Names
protease, serine, 34
NCBI Official Symbol
Prss34
NCBI Official Synonym Symbols
Mcp11; mMcp-11; C130091P12
NCBI Protein Information
mastin; mast cell protease 11

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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