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NDN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6010493

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NDN, Polyclonal Antibody
Full Product Name
NDN, NT (NDN, Necdin)
Product Gene Name
anti-NDN antibody
Product Synonym Names
Anti -NDN, NT (NDN, Necdin)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
36,086 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (23930554..23932450, complement). Location: 15q11.2-q12
OMIM
176270
3D Structure
ModBase 3D Structure for Q99608
Immunogen
NDN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human NDN.

Product Categories/Family for anti-NDN antibody

Antibodies; Abs to Disease Markers
Applications Tested/Suitable for anti-NDN antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-NDN antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-NDN antibody

Western Blot analysis in Ramos cell line lysates (35ug/lane) using MBS6010493. This demonstrates that MBS6010493 detected the NDN protein (arrow).
Rabbit Anti-NDN Polyclonal Antibody – Cat# MBS6010493

Flow Cytometry (FC/FACS) of anti-NDN antibody

Flow Cytometric analysis of Ramos cells using MBS6010493 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NDN Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6010493

Other Notes

Small volumes of anti-NDN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDN purchase

MBS6010493 is an antibody targeting NDN. Also known as NDN, NT (NDN, Necdin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NDN. It may not necessarily be applicable to this product.

NCBI GI #
4505349
NCBI GeneID
4692
NCBI Accession #
NP_002478.1
UniProt Primary Accession #
Q99608
UniProt Secondary Accession #
B2R6Z5
UniProt Related Accession #
Q99608
GenBank Nucleotide #
NM_002487.2
NCBI Official Full Name
necdin
NCBI Official Synonym Full Names
necdin, melanoma antigen (MAGE) family member
NCBI Official Symbol
NDN
NCBI Official Synonym Symbols
PWCR; HsT16328
UniProt Protein Name
Necdin
Protein Family
Necdin
UniProt Gene Name
NDN
UniProt Entry Name
NECD_HUMAN
NCBI Protein Information
necdin; necdin homolog; Prader-Willi syndrome chromosome region

UniProt Comments for NDN

NDN: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q11.2-q12

Cellular Component: centrosome; cell projection; perikaryon; cytosol; nucleus

Molecular Function: gamma-tubulin binding; DNA binding

Biological Process: nervous system development; central nervous system development; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; axon extension; neuron migration; axonal fasciculation; multicellular organismal homeostasis; sensory perception of pain; glial cell migration; post-embryonic development; negative regulation of cell proliferation; respiratory system process; regulation of transcription, DNA-dependent; regulation of growth

Disease: Prader-willi Syndrome
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Research Articles on NDN

1. Necdin expression declined during replicative aging of IMR90 primary human fibroblasts or after induction of premature senescence.Showed that in normal human cells, Necdin expression mimicked the effect of p53 inactivation by increasing radioresistance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDN antibody

 Reference Product  PubMed Publications
 E2F1 antibody  >3 publications with NDN and E2F1
 HIF1A antibody  >2 publications with NDN and HIF1A

Pathways associated with anti-NDN antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 P75(NTR)-mediated Signaling Pathway antibodies  P75(NTR)-mediated Signaling Pathway Diagram

Diseases associated with anti-NDN antibody

 Disease Name  Pubmed Publications
 Prader-Willi Syndrome Antibodies  >47 publications with NDN and Prader-Willi Syndrome
 Neurobehavioral Manifestations Antibodies  >36 publications with NDN and Neurobehavioral Manifestations
 Abnormalities, Multiple Antibodies  >36 publications with NDN and Abnormalities, Multiple
 Neoplasms Antibodies  >21 publications with NDN and Neoplasms
 Carcinoma Antibodies  >12 publications with NDN and Carcinoma
 Kidney Diseases Antibodies  >10 publications with NDN and Kidney Diseases
 Cardiovascular Diseases Antibodies  >4 publications with NDN and Cardiovascular Diseases
 Hypertension Antibodies  >4 publications with NDN and Hypertension
 Breast Neoplasms Antibodies  >4 publications with NDN and Breast Neoplasms
 Immune System Diseases Antibodies  >4 publications with NDN and Immune System Diseases

Organs/Tissues associated with anti-NDN antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >36 publications with NDN and Brain
 Blood Antibodies  >22 publications with NDN and Blood
 Embryonic Tissue Antibodies  >20 publications with NDN and Embryonic Tissue
 Kidney Antibodies  >10 publications with NDN and Kidney
 Muscle Antibodies  >9 publications with NDN and Muscle
 Lung Antibodies  >7 publications with NDN and Lung
 Testis Antibodies  >4 publications with NDN and Testis
 Placenta Antibodies  >4 publications with NDN and Placenta
 Bone Antibodies  >2 publications with NDN and Bone
 Pituitary Gland Antibodies  >2 publications with NDN and Pituitary Gland
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