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RASGRP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6010631

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can… More details

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Product Overview

Online Datasheet in PDF
Product Name
RASGRP2, Polyclonal Antibody
Full Product Name
RASGRP2, NT (RASGRP2, CDC25L, MCG7, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein)
Product Gene Name
anti-RASGRP2 antibody
Product Synonym Names
Anti -RASGRP2, NT (RASGRP2, CDC25L, MCG7, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
69,248 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (64494383..64512928, complement). Location: 11q13
OMIM
605577
3D Structure
ModBase 3D Structure for Q7LDG7
Immunogen
RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RASGRP2.

Product Categories/Family for anti-RASGRP2 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-RASGRP2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RASGRP2 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-RASGRP2 antibody

RASGRP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).
Rabbit Anti-RASGRP2 Polyclonal Antibody – Cat# MBS6010631

Immunohistochemistry (IHC) of anti-RASGRP2 antibody

RASGRP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-RASGRP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6010631

Immunofluorescence (IF) of anti-RASGRP2 antibody

Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-RASGRP2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6010631

Other Notes

Small volumes of anti-RASGRP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RASGRP2 purchase

MBS6010631 is an antibody targeting RASGRP2. Also known as RASGRP2, NT (RASGRP2, CDC25L, MCG7, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RASGRP2. It may not necessarily be applicable to this product.

NCBI GI #
149158729
NCBI GeneID
10235
NCBI Accession #
NP_001092141.1
UniProt Primary Accession #
Q7LDG7
UniProt Secondary Accession #
O00538; Q9UL65
UniProt Related Accession #
Q7LDG7
GenBank Nucleotide #
NM_001098671.1
NCBI Official Full Name
RAS guanyl-releasing protein 2
NCBI Official Synonym Full Names
RAS guanyl releasing protein 2 (calcium and DAG-regulated)
NCBI Official Symbol
RASGRP2
NCBI Official Synonym Symbols
CDC25L; CALDAG-GEFI
UniProt Protein Name
RAS guanyl-releasing protein 2
UniProt Synonym Protein Names
Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B3.3 kinase-like protein
Protein Family
RAS guanyl-releasing protein
UniProt Gene Name
RASGRP2
UniProt Synonym Gene Names
CDC25L; MCG7; CalDAG-GEFI; hCDC25L
UniProt Entry Name
GRP2_HUMAN
NCBI Protein Information
RAS guanyl-releasing protein 2; hCDC25L; cdc25-like protein; F25B3.3 kinase-like protein; guanine exchange factor MCG7; RAS guanyl nucleotide-releasing protein 2; calcium and DAG-regulated guanine nucleotide exchange factor I; calcium and diacylglycerol-regulated guanine nucleotide exchange factor I

UniProt Comments for RASGRP2

RASGRP2 iso2: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. Belongs to the RASGRP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: neuron projection; plasma membrane; synapse; cell junction; cytosol

Molecular Function: diacylglycerol binding; guanyl-nucleotide exchange factor activity; calcium ion binding; lipid binding

Biological Process: platelet activation; Ras protein signal transduction; regulation of cell growth; blood coagulation; signal transduction

Disease: Bleeding Disorder, Platelet-type, 18
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Research Articles on RASGRP2

1. analyzed the 5'-flanking region of rasgrp2 gene by a luciferase assay, which revealed that not only a promoter but also silencer regions were present upstream of D1E, suggesting rasgrp2 expression is controlled by a combination of promotion and repression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RASGRP2 antibody

 Reference Product  PubMed Publications
 RAP1B antibody  >3 publications with RASGRP2 and RAP1B
 RAP1A antibody  >1 publications with RASGRP2 and RAP1A

Pathways associated with anti-RASGRP2 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 FCERI Mediated NF-kB Activation Pathway antibodies  FCERI Mediated NF-kB Activation Pathway Diagram
 Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies  Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-RASGRP2 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >4 publications with RASGRP2 and Cardiovascular Diseases
 Neoplasms Antibodies  >4 publications with RASGRP2 and Neoplasms
 Disease Models, Animal Antibodies  >3 publications with RASGRP2 and Disease Models, Animal
 Hemorrhage Antibodies  >2 publications with RASGRP2 and Hemorrhage
 Nerve Degeneration Antibodies  >1 publications with RASGRP2 and Nerve Degeneration
 Nervous System Diseases Antibodies  >1 publications with RASGRP2 and Nervous System Diseases
 Congenital Abnormalities Antibodies  >1 publications with RASGRP2 and Congenital Abnormalities
 Brain Diseases Antibodies  >1 publications with RASGRP2 and Brain Diseases
 Lung Diseases Antibodies  >1 publications with RASGRP2 and Lung Diseases
 Inflammation Antibodies  >1 publications with RASGRP2 and Inflammation

Organs/Tissues associated with anti-RASGRP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >19 publications with RASGRP2 and Blood
 Vascular Antibodies  >9 publications with RASGRP2 and Vascular
 Muscle Antibodies  >2 publications with RASGRP2 and Muscle
 Bone Antibodies  >2 publications with RASGRP2 and Bone
 Brain Antibodies  >2 publications with RASGRP2 and Brain
 Kidney Antibodies  >1 publications with RASGRP2 and Kidney
 Lung Antibodies  >1 publications with RASGRP2 and Lung
 Eye Antibodies  >1 publications with RASGRP2 and Eye
 Bone Marrow Antibodies  >1 publications with RASGRP2 and Bone Marrow
 Connective Tissue Antibodies  >1 publications with RASGRP2 and Connective Tissue
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