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OATP2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601066

Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. The OATP family of proteins share significant sequence homology, and… More details

$1010 / 5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OATP2, 8, Monoclonal Antibody
Full Product Name
OATP2, 8 (Organic Anion Transporting Polypeptide 2, 8)
Product Synonym Names
Anti -OATP2, 8 (Organic Anion Transporting Polypeptide 2, 8)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5F260
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Purity/Purification
Supernatant
Hybridoma culture supernatant
Specificity
Recognizes OATP2 and OATP8 human organic anion transporters (90kD, 120kD, respectively, after SDS-PAGE). Recognizes antigen in transfected HEK293 cells. Does not recognize antigen in HepG2 or PLC cell lines.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 24aa from the N-terminus of human OATP2 conjugated to KLH.
Applications Tested/Suitable for anti-OATP2, 8 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-OATP2, 8 antibody
Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation.
Dilution: Immunohistochemistry (Paraffin sections): Requires microwave pretreatment. Incubate overnight at 4 degree C.
Immunofluorescence: Stains the sinusoidal (basolateral) membranes of human liver and hepatocelular carcinoma. Incubate for 1 hour at RT.
Western Blot: For optimal detection of the glycosylated antigen, sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37 degree C for 30 minutes.

Other Notes

Small volumes of anti-OATP2, 8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OATP2, 8 purchase

MBS601066 is an antibody targeting OATP2, 8. Also known as OATP2, 8 (Organic Anion Transporting Polypeptide 2, 8). Reactive in Human. Host: Mouse.
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