Mouse Anti-SLAMF6 Monoclonal Antibody – Cat# MBS601068

Detection of NTB‑A/SLAMF6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with MBS601068 (filled histogram) or isotype control antibody (open histogram).

SLAMF6 Antibody – Mouse Monoclonal

Name: SLAMF6 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601068
Price: 940 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601068

NTB-A is a type I transmembrane protein belonging to the SLAM family of immune cell receptors and has been designated SLAMF6. The SLAM family proteins are members of the CD2 subgroup of the Ig superfamily. NTB-A is expressed on NK-, T-, and B cells. NTB-A… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NTB-A (SLAMF6), Monoclonal Antibody

Full Product Name

NTB-A (SLAMF6) (PE)

Product Gene Name

anti-SLAMF6 antibody

Product Synonym Names

Anti -NTB-A (SLAMF6) (PE)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

7H16

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid. Labeled with R-Phycoerythrin (PE).

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography

Specificity

Recognizes human NTB-A.

Preparation and Storage

May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Molecular Weight

37,345 Da

Chromosome Location

chromosome: 1; Location: 1q23.2

OMIM

606446

3D Structure

ModBase 3D Structure for Q96DU3

Immunogen

Recombinant corresponding to aa28-225 of human NTB expressed in NSO cells.

Product Categories/Family for anti-SLAMF6 antibody

Antibodies; Abs to Receptors

Applications Tested/Suitable for anti-SLAMF6 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-SLAMF6 antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1x10e5 cells
Flow Cytometry Protocol: 1. Cells should be Fc-blocked by treatment with 1ug of human IgG/10e5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction.
2. Transfer 25ul of the Fc-blocked cells (1x10e5 cells) or 50ul of packed whole blood to a 5ml tube.
3. Add 10ul of N6000-35.
4. Incubate for 30-45 minutes at 4 degree C.
5. Following this incubation, remove unreacted NTB- A/SLAMF6 reagent by washing the cells twice in 4ml of the same PBS buffer (Note: whole blood will require an RBC lysis step at this point using any commercially available lysing reagent).
6. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis.
7. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2a antibody.

Other Notes

Small volumes of anti-SLAMF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLAMF6 purchase

MBS601068 is an antibody targeting SLAMF6. Also known as NTB-A (SLAMF6) (PE). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SLAMF6. It may not necessarily be applicable to this product.

NCBI GI #

15384843

NCBI GeneID

114836

UniProt Primary Accession #

Q96DU3

UniProt Secondary Accession #

Q14CF0; Q5TAS4; Q5TAT3; Q96DV0; A6NMW2

UniProt Related Accession #

Q96DU3

NCBI Official Full Name

NTB-A receptor

NCBI Official Synonym Full Names

SLAM family member 6

NCBI Official Symbol

SLAMF6

NCBI Official Synonym Symbols

KALI; NTBA; KALIb; Ly108; NTB-A; SF2000; FLJ50657; MGC104953; SLAMF6

UniProt Protein Name

SLAM family member 6

UniProt Synonym Protein Names

Activating NK receptor; NK-T-B-antigen

Protein Family

SLAM family

UniProt Gene Name

SLAMF6

UniProt Synonym Gene Names

KALI

UniProt Entry Name

SLAF6_HUMAN

NCBI Protein Information

SLAM family member 6; NTBA receptor; NK-T-B-antigen; OTTHUMP00000024349; activating NK receptor; natural killer-, T- and B-cell antigen

NCBI Summary for SLAMF6

The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Comments for SLAMF6

SLAMF6: Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: receptor activity

Research Articles on SLAMF6

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
2. Observational study of gene-disease association. (HuGE Navigator)
3. 2B4, NTB-A and CRACC have roles in the regulation of Natural Killer cell function [review]
4. HTLV-1-infected CD4+ T cells did not express ligands for NK cell activating receptors, NCR and NKG2D, although they did express ligands for NK cell coactivating receptors, NTB-A and 2B4.
5. The 3.0 A crystal structure of the complete NTB-A ectodomain revealed a rod-like monomer that self-associates to form a highly kinked dimer spanning an end-to-end distance of approximately 100 A.
6. regulation of interferon-gamma secretion, and not interleukin-4 in vitro, as well as inhibition of Th1 cell-induced isotype switching and attenuation of experimental allergic encephalomyelitis identifies NTB-A as a regulator of T cell response
7. blocking the engagement of 2B4, NTB-A and CRACC has no effect on the proliferation or development of the cytotoxic potential of NK cells but triggering by their physiological ligands on MHC class I-negative target cells induces potent NK cell cytotoxicity
8. NTB-A is an interlymphocyte signaling molecule, which serves to orchestrate the activities of immune cells.
9. NTB-A-mediated IFN-gamma production was greatly reduced in the absence of SLAM-associated protein (SAP), demonstrating that cytokine production and cytotoxicity are differentially dependent on SAP and possibly EAT-2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLAMF6 antibody

Reference Product	PubMed Publications
SH2D1A antibody	>1 publications with SLAMF6 and SH2D1A

Diseases associated with anti-SLAMF6 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with SLAMF6 and Neoplasms
Lymphoma, T-Cell Antibodies	>1 publications with SLAMF6 and Lymphoma, T-Cell

Organs/Tissues associated with anti-SLAMF6 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7 publications with SLAMF6 and Blood
Bone Marrow Antibodies	>5 publications with SLAMF6 and Bone Marrow
Thymus Antibodies	>2 publications with SLAMF6 and Thymus
Pharynx Antibodies	>2 publications with SLAMF6 and Pharynx
Spleen Antibodies	>1 publications with SLAMF6 and Spleen