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B3GALT5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6010891

This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and… More details

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Product Overview

Online Datasheet in PDF
Product Name
B3GALT5, Polyclonal Antibody
Full Product Name
B3GALT5, NT (B3GALT5, Beta-1,3-galactosyltransferase 5, Beta-3-Gx-T5, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5)
Product Gene Name
anti-B3GALT5 antibody
Product Synonym Names
Anti -B3GALT5, NT (B3GALT5, Beta-1,3-galactosyltransferase 5, Beta-3-Gx-T5, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Concentration
0.24mg/mL (lot specific)
Specificity
Recognizes human B3GALT5.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 21; NC_000021.8 (40928369..41034816). Location: 21q22.3
OMIM
604066
3D Structure
ModBase 3D Structure for Q9Y2C3
Immunogen
B3GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-91 amino acids from the N-terminal region of human B3GALT5.

Product Categories/Family for anti-B3GALT5 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase
Applications Tested/Suitable for anti-B3GALT5 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC)
Application Notes for anti-B3GALT5 antibody
EIA: 1:1,000
WB: 1:100-500
Indirect Flow Cytometry: 1:10-50

Western Blot (WB) of anti-B3GALT5 antibody

B3GALT5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the B3GALT5 antibody detected the B3GALT5 protein (arrow).
Rabbit Anti-B3GALT5 Polyclonal Antibody – Cat# MBS6010891

Flow Cytometry (FC) of anti-B3GALT5 antibody

B3GALT5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-B3GALT5 Polyclonal Antibody, Flow Cytometry (FC) – Cat# MBS6010891

Other Notes

Small volumes of anti-B3GALT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B3GALT5 purchase

MBS6010891 is an antibody targeting B3GALT5. Also known as B3GALT5, NT (B3GALT5, Beta-1,3-galactosyltransferase 5, Beta-3-Gx-T5, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for B3GALT5. It may not necessarily be applicable to this product.

NCBI GI #
520975380
NCBI GeneID
10317
NCBI Accession #
NP_001265579.1
UniProt Primary Accession #
Q9Y2C3
UniProt Secondary Accession #
Q2M3L5; Q53Z19; Q9NY96; Q9P1X6; Q9P1X7; A8KA86; D3DSI3
UniProt Related Accession #
Q9Y2C3
GenBank Nucleotide #
NM_001278650.1
NCBI Official Full Name
beta-1,3-galactosyltransferase 5 isoform a
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
NCBI Official Symbol
B3GALT5
NCBI Official Synonym Symbols
B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
UniProt Protein Name
Beta-1,3-galactosyltransferase 5
UniProt Synonym Protein Names
Beta-3-Gx-T5; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5
Protein Family
Beta-1,3-galactosyltransferase
UniProt Gene Name
B3GALT5
UniProt Synonym Gene Names
Beta-1,3-GalTase 5; Beta3Gal-T5; Beta3GalT5; b3Gal-T5
UniProt Entry Name
B3GT5_HUMAN
NCBI Protein Information
beta-1,3-galactosyltransferase 5; beta-3-galactosyltransferase 5; homolog of C. elegans Bt toxin resistance gene bre-5; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5

NCBI Summary for B3GALT5

This gene encodes a member of a family of membrane-bound glycoproteins. The encoded protein may synthesize type 1 Lewis antigens, which are elevated in gastrointestinal and pancreatic cancers. Alternatively spliced transcript variants have been observed for this gene, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2013]
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UniProt Comments for B3GALT5

B3GALT5: Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Belongs to the glycosyltransferase 31 family.

Protein type: Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum; integral to membrane

Molecular Function: UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity

Biological Process: protein amino acid glycosylation
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Research Articles on B3GALT5

1. Chromatin immunoprecipitation assays on beta3Gal-T5 promoter showed that histone H3K4 trymethylation, H3K79 dimethylation, and H3K9-14 acetylation are high in cells expressing the transcript.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-B3GALT5 antibody

 Reference Product  PubMed Publications
 FUT2 antibody  >2 publications with B3GALT5 and FUT2
 FUT3 antibody  >1 publications with B3GALT5 and FUT3
 FUT1 antibody  >1 publications with B3GALT5 and FUT1

Pathways associated with anti-B3GALT5 antibody

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Biosynthesis - Globo Series Pathway antibodies  Glycosphingolipid Biosynthesis - Globo Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Globo Series Pathway antibodies  Glycosphingolipid Biosynthesis - Globo Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis, Lacto-series, LacCer => Lc4Cer Pathway antibodies  Glycosphingolipid Biosynthesis, Lacto-series, LacCer => Lc4Cer Pathway Diagram
 Glycosphingolipid Biosynthesis, Lacto-series, LacCer => Lc4Cer Pathway antibodies  Glycosphingolipid Biosynthesis, Lacto-series, LacCer => Lc4Cer Pathway Diagram

Diseases associated with anti-B3GALT5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7 publications with B3GALT5 and Neoplasms
 Gastrointestinal Diseases Antibodies  >4 publications with B3GALT5 and Gastrointestinal Diseases
 Uterine Cervical Neoplasms Antibodies  >1 publications with B3GALT5 and Uterine Cervical Neoplasms
 Stomach Neoplasms Antibodies  >1 publications with B3GALT5 and Stomach Neoplasms
 Breast Neoplasms Antibodies  >1 publications with B3GALT5 and Breast Neoplasms
 Carcinoma, Squamous Cell Antibodies  >1 publications with B3GALT5 and Carcinoma, Squamous Cell
 Inflammation Antibodies  >1 publications with B3GALT5 and Inflammation

Organs/Tissues associated with anti-B3GALT5 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >4 publications with B3GALT5 and Intestine
 Mammary Gland Antibodies  >1 publications with B3GALT5 and Mammary Gland
 Testis Antibodies  >1 publications with B3GALT5 and Testis
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