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Integrin Alphav, Beta5 (ITGB5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601117

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and… More details

$845 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Integrin alphaV, beta5 (ITGB5), Monoclonal Antibody
Full Product Name
Integrin alphaV, beta5
Product Gene Name
anti-ITGB5 antibody
Product Synonym Names
Anti -Integrin alphaV, beta5
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2Q873
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS. No preservative added.
Purity/Purification
Purified
Concentration
1mg/ml (lot specific)
Specificity
Recognizes human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
88,054 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (124480795..124606500, complement). Location: 3q21.2
OMIM
147561
3D Structure
ModBase 3D Structure for P18084
Immunogen
Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.

Product Categories/Family for anti-ITGB5 antibody

Antibodies; Abs to Integrin
Applications Tested/Suitable for anti-ITGB5 antibody
ELISA (EL/EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-ITGB5 antibody
Recommended Dilutions:
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4°( in 20% normal human serum then washing in medium containing 1 00/0 FRS~ 5x 1 Oe5 cells per tube were subsequently incubated with primary antibody for 30 minutes on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody. Immunocytochemistry: 1 Smg/ml for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1 % paraformaldehyde containing 0.5% Triton X-l 00 for 20 minutes. Incubation for 30 minutes at 22°(, followed by detection with a fluorescein-conjugated secondary antibody. Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of anti-ITGB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGB5 purchase

MBS601117 is an antibody targeting ITGB5. Also known as Integrin alphaV, beta5. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGB5. It may not necessarily be applicable to this product.

NCBI GI #
20127446
NCBI GeneID
3693
NCBI Accession #
NP_002204.2
UniProt Primary Accession #
P18084
UniProt Secondary Accession #
B0LPF8; B2RD70
UniProt Related Accession #
P18084
GenBank Nucleotide #
NM_002213.3
NCBI Official Full Name
integrin beta-5
NCBI Official Synonym Full Names
integrin, beta 5
NCBI Official Symbol
ITGB5
UniProt Protein Name
Integrin beta-5
Protein Family
Integrin
UniProt Gene Name
ITGB5
UniProt Entry Name
ITB5_HUMAN
NCBI Protein Information
integrin beta-5

UniProt Comments for ITGB5

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: focal adhesion; cell surface; leading edge; plasma membrane; phagocytic vesicle; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; extracellular matrix organization and biogenesis; muscle contraction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
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Research Articles on ITGB5

1. Depletion of integrin beta5 in triple-negative breast carcinoma cells markedly reduced tumor take, growth and tumor angiogenesis, whereas reexpression of integrin beta5 rescued this phenotype; findings show a critical role for integrin beta5 in the tumorigenic potential of breast carcinoma cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGB5 antibody

 Reference Product  PubMed Publications
 ITGAV antibody  >5 publications with ITGB5 and ITGAV
 ITGA3 antibody  >3 publications with ITGB5 and ITGA3
 ITGA5 antibody  >3 publications with ITGB5 and ITGA5
 PTK2 antibody  >2 publications with ITGB5 and PTK2
 ITGA1 antibody  >1 publications with ITGB5 and ITGA1
 TLN1 antibody  >1 publications with ITGB5 and TLN1
 VTN antibody  >1 publications with ITGB5 and VTN

Pathways associated with anti-ITGB5 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway antibodies  Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram

Diseases associated with anti-ITGB5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >9 publications with ITGB5 and Neoplasms
 Inflammation Antibodies  >3 publications with ITGB5 and Inflammation
 Neoplasm Metastasis Antibodies  >2 publications with ITGB5 and Neoplasm Metastasis
 Anemia Antibodies  >1 publications with ITGB5 and Anemia
 Breast Neoplasms Antibodies  >1 publications with ITGB5 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with ITGB5 and Neoplasms, Experimental
 Lung Diseases Antibodies  >1 publications with ITGB5 and Lung Diseases

Organs/Tissues associated with anti-ITGB5 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with ITGB5 and Blood
 Uterus Antibodies  >4 publications with ITGB5 and Uterus
 Placenta Antibodies  >4 publications with ITGB5 and Placenta
 Vascular Antibodies  >3 publications with ITGB5 and Vascular
 Muscle Antibodies  >2 publications with ITGB5 and Muscle
 Bone Marrow Antibodies  >2 publications with ITGB5 and Bone Marrow
 Stomach Antibodies  >2 publications with ITGB5 and Stomach
 Bone Antibodies  >2 publications with ITGB5 and Bone
 Lung Antibodies  >2 publications with ITGB5 and Lung
 Prostate Antibodies  >1 publications with ITGB5 and Prostate
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