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SPAG4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6011199

May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPAG4, Monoclonal Antibody
Full Product Name
SPAG4 (Sperm-associated Antigen 4 Protein, Outer Dense Fiber-associated Protein SPAG4, SUN Domain-containing Protein 4, SUN4)
Product Gene Name
Product Synonym Names
Anti -SPAG4 (Sperm-associated Antigen 4 Protein, Outer Dense Fiber-associated Protein SPAG4, SUN Domain-containing Protein 4, SUN4)
Immunogen Sequence
AELDKLHKEV STVRAANSER VAKLVFQRLN EDFVRKPDYA LSSVGASIDL QKTSHDYADR NTAYFWNRFS FWNYARPPTV ILEPHVFPGN CWAFEGDQGQ VVIQLPGRVQ *
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3C8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SPAG4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
48,165 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (34203776..34208967). Location: 20q11.21
OMIM
603038
3D Structure
ModBase 3D Structure for Q9NPE6
Immunogen
Partial recombinant corresponding to aa221-331 from human SPAG4 (NP_003107) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-SPAG4 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SPAG4 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-SPAG4 antibody

Western Blot detection against Immunogen (38.21kD).
Mouse Anti-SPAG4 Monoclonal Antibody – Cat# MBS6011199

Testing Data of anti-SPAG4 antibody

Detection limit for recombinant GST tagged SPAG4 is 1ng/ml as a capture antibody.
Mouse Anti-SPAG4 Monoclonal Antibody, Testing Data – Cat# MBS6011199

Other Notes

Small volumes of anti-SPAG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPAG4 purchase

MBS6011199 is an antibody targeting SPAG4. Also known as SPAG4 (Sperm-associated Antigen 4 Protein, Outer Dense Fiber-associated Protein SPAG4, SUN Domain-containing Protein 4, SUN4). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SPAG4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sperm-associated antigen 4 protein
NCBI Official Synonym Full Names
sperm associated antigen 4
NCBI Official Symbol
SPAG4
NCBI Official Synonym Symbols
SUN4; CT127
UniProt Protein Name
Sperm-associated antigen 4 protein
UniProt Synonym Protein Names
Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4
UniProt Gene Name
SPAG4
UniProt Synonym Gene Names
SUN4
UniProt Entry Name
SPAG4_HUMAN
NCBI Protein Information
sperm-associated antigen 4 protein; sperm tail protein; acrosomal protein ACR55; cancer/testis antigen 127; SUN domain-containing protein 4; Sad1 and UNC84 domain containing 4; outer dense fiber-associated protein SPAG4

NCBI Summary for SPAG4

The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPAG4

SPAG4: May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.

Protein type: Membrane protein, integral; Microtubule-binding; Cancer Testis Antigen (CTA); Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: cytoskeleton; cytoplasm; integral to membrane; nuclear envelope

Molecular Function: structural molecule activity

Biological Process: spermatogenesis; nuclear membrane organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPAG4 antibody

Organs/Tissues associated with anti-SPAG4 antibody

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