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F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6011342

Coagulation Factor IX (F9) circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC), Monoclonal Antibody
Full Product Name
F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC)
Product Synonym Names
Anti -F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC)
Immunogen Sequence
QCESNPCLNG GSCKDDINSY ECWCPFGFEG KNCELDVTCN IKNGRCEQFC KNSADNKVVC SCTEGYRLAE NQKSCEPAVP FPCGRVSVSQ TSKLT
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2C9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human F9.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa96-190 from human F9 (NP_000124) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody

Antibodies; Abs to Coagulation Factors
Applications Tested/Suitable for anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody
Suitable for use in ELISA, Western Blot and Immunoprecipitation.

Western Blot (WB) of anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody

Western Blot detection against Immunogen (36.19kD).
Mouse Anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) Monoclonal Antibody – Cat# MBS6011342

Western Blot (WB) of anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody

Western Blot analysis of F9 expression in transfected 293T cell line by MBS6011342 Lane 1: F9 transfected lysate (51.8kD).|Lane 2: Non-transfected lysate.
Mouse Anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) Monoclonal Antibody, Western Blot (WB) – Cat# MBS6011342

Immunoprecipitation (IP) of anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody

Immunoprecipitation of F9 transfected lysate using MBS6011342 and Protein A Magnetic Bead and immunoblotted with F9 rabbit polyclonal antibody.
Mouse Anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6011342

Testing Data of anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody

Detection limit for recombinant GST tagged F9 is ~0.1ng/ml as a capture antibody.
Mouse Anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) Monoclonal Antibody, Testing Data – Cat# MBS6011342

Western Blot (WB) of anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody

Western blot analysis of F9 over-expressed 293 cell line, cotransfected with F9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MBS6011342. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) Monoclonal Antibody, Western Blot (WB) – Cat# MBS6011342

Other Notes

Small volumes of anti-F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC) purchase

MBS6011342 is an antibody targeting F9 (Coagulation Factor IX, Christmas Factor, Plasma Thromboplastin Component, PTC). Reactive in Human. Host: Mouse.
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