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Caldesmon, LMW Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601143

Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The… More details

$760 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Caldesmon, LMW, Monoclonal Antibody
Full Product Name
Caldesmon, LMW (BSA & Azide Free)
Product Synonym Names
Anti -Caldesmon, LMW (BSA & Azide Free)
Immunogen Sequence
Not determined
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F41
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C1032-40
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes Caldesmon, LMW. Species Crossreactivity: human.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
80kD (l-caldesmon)
Immunogen
Recombinant caldesmon
Cellular Localization
Cytoplasmic
Positive Control
Rh30 cells. Uterus

Product Categories/Family for anti-Caldesmon, LMW antibody

Antibodies; Abs to Caldesmon
Applications Tested/Suitable for anti-Caldesmon, LMW antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Caldesmon, LMW antibody
Dilution: Immunofluorescence
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Western Blot (WB) of anti-Caldesmon, LMW antibody

Stain|Western Blot
Mouse Anti-Caldesmon, LMW Monoclonal Antibody – Cat# MBS601143

Immunohistochemistry (IHC) of anti-Caldesmon, LMW antibody

Formalin-fixed, paraffin-embedded human uterus stained with Caldesmon Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.
Mouse Anti-Caldesmon, LMW Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS601143

Other Notes

Small volumes of anti-Caldesmon, LMW antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Caldesmon, LMW purchase

MBS601143 is an antibody targeting Caldesmon, LMW. Also known as Caldesmon, LMW (BSA & Azide Free). Reactive in Human. Host: Mouse.
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