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SPO11 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6011440

MBS6011440 is an antibody targeting SPO11. Also known as SPO11 (Meiotic Recombination Protein SPO11, Cancer/testis Antigen 35, CT35). Reactive in Human. Host: Rabbit. More details

$970 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPO11, Polyclonal Antibody
Full Product Name
SPO11 (Meiotic Recombination Protein SPO11, Cancer/testis Antigen 35, CT35)
Product Gene Name
Product Synonym Names
Anti -SPO11 (Meiotic Recombination Protein SPO11, Cancer/testis Antigen 35, CT35)
Immunogen Sequence
MAFAPMGPEA SFFDVLDRHR ESLLAALRRG GREPPTGGSR LASRFEDSVG LQMVSHCTTR KIKSDSPKSA QKFSLILKIL SMIYKLVQSN TYATKRDIYY TDSQLFGNQT VVDNIINDIS CMLKVSRRSL HILSTSKGLI AGNLRYIEED GTKVNCTCGA TAVAVPSNIQ GIRNLVTDAK FVLIVEKDAT FQRLLDDNFC NKLSPCIMIT GKGVPDLNTR LLVKKLWDTF HVPVFTLVDA DPHGIEIMCI YKYGSMSMSF EAHHLTVPAI RWLGLLPSDL KRLNVPKDSL IPLTKRDQMK LDSILRRPYV TCQPFWRKEM EIMADSKMKA EIQALTFLSS DYLSRVYLPN KLKFGGWI
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SPO11.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
44,537 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (55904831..55919049). Location: 20q13.31
OMIM
605114
3D Structure
ModBase 3D Structure for Q9Y5K1
Immunogen
Full length human SPO11, aa1-358 (NP_937998.1).
Applications Tested/Suitable for anti-SPO11 antibody
Western Blot (WB)
Application Notes for anti-SPO11 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-SPO11 antibody

Western Blot analysis of SPO11 expression in transfected 293T cell line by SPO11 polyclonal antibody. Lane 1: SPO11 transfected lysate (40.4kD). Lane 2: Non-transfected lysate.
Rabbit Anti-SPO11 Polyclonal Antibody – Cat# MBS6011440

Other Notes

Small volumes of anti-SPO11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPO11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
SPO11
NCBI Official Synonym Full Names
SPO11 meiotic protein covalently bound to DSB
NCBI Official Symbol
SPO11
NCBI Official Synonym Symbols
CT35; TOPVIA; SPATA43
UniProt Protein Name
Meiotic recombination protein SPO11
UniProt Synonym Protein Names
Cancer/testis antigen 35
UniProt Gene Name
SPO11
UniProt Synonym Gene Names
CT35
UniProt Entry Name
SPO11_HUMAN
NCBI Protein Information
meiotic recombination protein SPO11; cancer/testis antigen 35; spermatogenesis associated 43; SPO11 meiotic protein covalently bound to DSB homolog

NCBI Summary for SPO11

Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPO11

SPO11: Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. Belongs to the TOP6A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cell development/differentiation; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: chromosome, telomeric region; nucleus

Molecular Function: DNA binding; hydrolase activity; ATP binding

Biological Process: ovarian follicle development; male meiosis I; meiotic recombination; synapsis; female gamete generation; spermatogenesis; DNA catabolic process, endonucleolytic; spermatid development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SPO11 antibody

Organs/Tissues associated with anti-SPO11 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with SPO11 and Brain
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