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SIKE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6011464

SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SIKE (SIKE1), Polyclonal Antibody
Full Product Name
SIKE, CT (SIKE1, SIKE, Suppressor of IKBKE 1, Suppressor of IKK-epsilon)
Product Gene Name
Product Synonym Names
Anti -SIKE, CT (SIKE1, SIKE, Suppressor of IKBKE 1, Suppressor of IKK-epsilon)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
23,721 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (115312100..115323308, complement). Location: 1p13.2
OMIM
611656
3D Structure
ModBase 3D Structure for Q9BRV8
Immunogen
SIKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the C-terminal region of human SIKE.

Product Categories/Family for anti-SIKE1 antibody

Applications Tested/Suitable for anti-SIKE1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SIKE1 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-SIKE1 antibody

SIKE Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIKE antibody detected the SIKE protein (arrow).
Rabbit Anti-SIKE1 Polyclonal Antibody – Cat# MBS6011464

Immunohistochemistry (IHC) of anti-SIKE1 antibody

SIKE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIKE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-SIKE1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6011464

Other Notes

Small volumes of anti-SIKE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SIKE1 purchase

MBS6011464 is an antibody targeting SIKE1. Also known as SIKE, CT (SIKE1, SIKE, Suppressor of IKBKE 1, Suppressor of IKK-epsilon). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SIKE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
suppressor of IKBKE 1 isoform 1
NCBI Official Synonym Full Names
suppressor of IKBKE 1
NCBI Official Symbol
SIKE1
NCBI Official Synonym Symbols
SIKE; RP5-1000E10.4
UniProt Protein Name
Suppressor of IKBKE 1
UniProt Synonym Protein Names
Suppressor of IKK-epsilon
Protein Family
UniProt Gene Name
SIKE1
UniProt Synonym Gene Names
SIKE
UniProt Entry Name
SIKE1_HUMAN
NCBI Protein Information
suppressor of IKBKE 1; suppressor of IKK epsilon; suppressor of IKK-epsilon

UniProt Comments for SIKE1

SIKE: Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways. Belongs to the SIKE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytosol

Molecular Function: protein binding

Biological Process: innate immune response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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