Rabbit Anti-PEK Monoclonal Antibody – Cat# MBS601178

Western Blot of extracts from AR42J cells untreated (-) or treated (+) with 1uM thapsigargin (tg) for 20 minutes using MBS601178.

PERK (PEK) Antibody – Rabbit Monoclonal

Name: PEK Antibody; Monoclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601178
Price: 865 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601178

PERK, an eIF2 alpha kinase, is a transmembrane protein resident in the endoplasmic reticulum (ER) membrane and couples ER stress signals to translation inhibition. ER stress increases the activity of PERK, which then phosphorylates eIF2 alpha, resulting in… More details

$865 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PERK (PEK), Monoclonal Antibody

Full Product Name

PERK, phosphorylated (Thr980) (PKR-like ER Resident Kinase)

Product Gene Name

anti-PEK antibody

Product Synonym Names

Anti -PERK, phosphorylated (Thr980) (PKR-like ER Resident Kinase)

Clonality

Monoclonal

Isotype

IgG

Clone Number

7H50

Host

Rabbit

Species Reactivity

Rat

Form/Format

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

Purity/Purification

Purified

Specificity

Recognizes endogenous levels of PERK phosphorylated at Thr980 at ~170kD. Species Crossreactivity: rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

~170kd

Chromosome Location

Chromosome: 3R; NT_033777.2 (1284285..1290475, complement). Location: 83A4-83A4

3D Structure

ModBase 3D Structure for Q9NIV1

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK, conjugated to KLH. Species Sequence Homology: mouse.

Grade

Purified

Product Categories/Family for anti-PEK antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-PEK antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-PEK antibody

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000. Incubate membranes with diluted antibody, 1x TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking overnight. Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of anti-PEK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEK purchase

MBS601178 is an antibody targeting PEK. Also known as PERK, phosphorylated (Thr980) (PKR-like ER Resident Kinase). Reactive in Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PEK. It may not necessarily be applicable to this product.

NCBI GI #

442617553

NCBI GeneID

40653

NCBI Accession #

NP_001262283.1

UniProt Primary Accession #

Q9NIV1

UniProt Secondary Accession #

Q8SX61; Q9VNB8

UniProt Related Accession #

Q9NIV1

GenBank Nucleotide #

NM_001275354.1

NCBI Official Full Name

pancreatic eIF-2alpha kinase, isoform C

NCBI Official Synonym Full Names

pancreatic eIF-2alpha kinase

NCBI Official Symbol

PEK

NCBI Official Synonym Symbols

CG2087; DmelCG2087; DmPEK; DpERK; DPERK; EIF2-like; perk; pERK; Perk; PERK

UniProt Protein Name

Eukaryotic translation initiation factor 2-alpha kinase

UniProt Synonym Protein Names

PRKR-like endoplasmic reticulum kinase

Protein Family

Eukaryotic translation initiation factor 2-alpha kinase

UniProt Gene Name

PEK

UniProt Synonym Gene Names

EIF2AK3; DmPEK; PEK; PERK

UniProt Entry Name

E2AK3_DROME

NCBI Protein Information

CG2087 gene product from transcript CG2087-RA; CG2087-PA; CG2087-PB; CG2087-PC; PEK-PA; PEK-PB; PEK-PC; PERK; pancreatic eIF-2alpha kinase; pancreatic eIF2alpha kinase

UniProt Comments for PEK

Function: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction

By similarity.

Subunit structure: Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein

By similarity.

Induction: By ER stress.

Domain: The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP

By similarity.

Post-translational modification: Autophosphorylated

By similarity.N-glycosylated

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.Contains 1 protein kinase domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PEK antibody

Reference Product	PubMed Publications
eIF-2alpha antibody	>17 publications with PEK and eIF-2alpha
wek antibody	>13 publications with PEK and wek
ire-1 antibody	>7 publications with PEK and ire-1
Atf6 antibody	>4 publications with PEK and Atf6
Xbp1 antibody	>2 publications with PEK and Xbp1

Pathways associated with anti-PEK antibody

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
PERK Regulated Gene Expression Pathway antibodies	PERK Regulated Gene Expression Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Translation Factors Pathway antibodies	Translation Factors Pathway Diagram
Unfolded Protein Response Pathway antibodies	Unfolded Protein Response Pathway Diagram

Organs/Tissues associated with anti-PEK antibody

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>8 publications with PEK and Embryonic Tissue
Whole Body Antibodies	>3 publications with PEK and Whole Body
Testis Antibodies	>1 publications with PEK and Testis