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EPPIN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6012194

MBS6012194 is an antibody targeting EPPIN. Also known as Eppin (Cancer/testis Antigen 71, CT71, Epididymal Protease Inhibitor, Protease Inhibitor WAP7, Serine Protease Inhibitor-like with Kunitz and WAP Domains 1, SPINLW1, WAP Four-disulfide Core Domain… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Eppin, Monoclonal Antibody
Full Product Name
Eppin (Cancer/testis Antigen 71, CT71, Epididymal Protease Inhibitor, Protease Inhibitor WAP7, Serine Protease Inhibitor-like with Kunitz and WAP Domains 1, SPINLW1, WAP Four-disulfide Core Domain Protein 7, WAP7, WFDC7)
Product Gene Name
Product Synonym Names
Anti -Eppin (Cancer/testis Antigen 71, CT71, Epididymal Protease Inhibitor, Protease Inhibitor WAP7, Serine Protease Inhibitor-like with Kunitz and WAP Domains 1, SPINLW1, WAP Four-disulfide Core Domain Protein 7, WAP7, WFDC7)
Immunogen Sequence
PGLTDWLFPR RCPKIREECE FQERDVCTKD RQCQDNKKCC VFSCGKKCLD LKQDVCEMPK ETGPCLAYFL HWWYDKKDNT CSMFVYGGCQ GNNNNFQSKA NCLNTCKNKR FP
Clonality
Monoclonal
Isotype
IgM,k
Clone Number
4G3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in ascites fluid.
Purity/Purification
Ascites
Ascites
Specificity
Recognizes human SPINLW1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
15,284 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (44169265..44176516, complement). Location: 20q13.12
OMIM
609031
3D Structure
ModBase 3D Structure for O95925
Immunogen
Partial recombinant corresponding to aa22-134 from human SPINLW1 (NP_065131) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-EPPIN antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-EPPIN antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-EPPIN antibody

Western Blot detection against Immunogen (38.43kD).
Mouse Anti-EPPIN Monoclonal Antibody – Cat# MBS6012194

Other Notes

Small volumes of anti-EPPIN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EPPIN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eppin
NCBI Official Synonym Full Names
epididymal peptidase inhibitor
NCBI Official Symbol
EPPIN
NCBI Official Synonym Symbols
CT71; CT72; WAP7; WFDC7; SPINLW1; dJ461P17.2
UniProt Protein Name
Eppin
UniProt Synonym Protein Names
Cancer/testis antigen 71; CT71; Epididymal protease inhibitor; Protease inhibitor WAP7; Serine protease inhibitor-like with Kunitz and WAP domains 1; WAP four-disulfide core domain protein 7
Protein Family
UniProt Gene Name
EPPIN
UniProt Synonym Gene Names
SPINLW1; WAP7; WFDC7; CT71
UniProt Entry Name
EPPI_HUMAN
NCBI Protein Information
eppin; protease inhibitor WAP7; cancer/testis antigen 71; cancer/testis antigen 72; epididymal protease inhibitor; WAP four-disulfide core domain protein 7; serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin)

NCBI Summary for EPPIN

This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. [provided by RefSeq, Nov 2010]
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UniProt Comments for EPPIN

Spinlw1: Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell surface; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding

Biological Process: defense response to bacterium; negative regulation of peptidase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EPPIN antibody

 Reference Product  PubMed Publications
 JUN antibody  >3 publications with EPPIN and JUN

Organs/Tissues associated with anti-EPPIN antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >18 publications with EPPIN and Testis
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