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Neurofilament L, 68kD Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6012347

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which include the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include other… More details

$720 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Neurofilament L, 68kD, Polyclonal Antibody
Full Product Name
Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)
Product Synonym Names
Anti -Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Porcine, Rat
Form/Format
Supplied as a liquid.
Purity/Purification
Serum
Serum
Specificity
Recognizes porcine and rat NF-L.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Porcine intermediate filaments were prepared from spinal cords by the method of Delacourte et al., and this cytoskeletal material was dissolved in 6M urea.
Applications Tested/Suitable for anti-Neurofilament L, 68kD antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.
Dilution: Immunocytochemistry (ABC): 1:5000
Immunofluorescence: 1:500-1:1000
Western Blot: 1:20,000

Western Blot (WB) of anti-Neurofilament L, 68kD antibody

Western Blot of whole rat cerebellum homogenate stained with MBS6012347 in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kD corresponds to rodent NF-L. Faint bands below this are in vivo degradation products generated by calpain digestion. The major NF-H band in human, cow and bovine run a little slower on SDS-PAGE, at about 70kD. Right lane shows a strip incubated with a new antibody against neuron specific enolase, which reveals a major band at 47kD.
Rabbit Anti-Neurofilament L, 68kD Polyclonal Antibody – Cat# MBS6012347

Western Blot (WB) of anti-Neurofilament L, 68kD antibody

Mixed neuron/glia cultures from newborn rat brain stained with 031420 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L MBS6012347 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA
Rabbit Anti-Neurofilament L, 68kD Polyclonal Antibody, Western Blot (WB) – Cat# MBS6012347

Other Notes

Small volumes of anti-Neurofilament L, 68kD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Neurofilament L, 68kD purchase

MBS6012347 is an antibody targeting Neurofilament L, 68kD. Also known as Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein). Reactive in Porcine, Rat. Host: Rabbit.
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