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Neuron Specific Nuclear Protein Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601235

Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. More details

$1105 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Neuron Specific Nuclear Protein, Monoclonal Antibody
Full Product Name
Neuron Specific Nuclear Protein (NeuN) (Biotin)
Product Synonym Names
Anti -Neuron Specific Nuclear Protein (NeuN) (Biotin)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H412
Host
Mouse
Species Reactivity
Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.1% sodium azide. Labeled with Biotin.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Neuron Specific Nuclear Protein (NeuN). Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Non-reactive cell types include Purkinje, mitral and photoreceptor cells. Species Crossreactivity: rat
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified cell nuclei from mouse brain. Species Sequence Homology: human, ferret, chick and salamander
Species Sequence Homology
Human, ferret, chick and salamander

Product Categories/Family for anti-Neuron Specific Nuclear Protein antibody

Antibodies; Abs to Neuroscience
Applications Tested/Suitable for anti-Neuron Specific Nuclear Protein antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-Neuron Specific Nuclear Protein antibody
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.
Dilution: Immunohistochemistry: 1:100; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde- based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.
Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD.
Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100.

Testing Data of anti-Neuron Specific Nuclear Protein antibody

Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 12 hours after treatment. The blue staining is DAPI. MBS601235 detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.
Mouse Anti-Neuron Specific Nuclear Protein Monoclonal Antibody – Cat# MBS601235

Testing Data of anti-Neuron Specific Nuclear Protein antibody

Immunolocalization of Neuron Specific Nuclear Protein (green) and Nestin (red) in trimethyl tin treated mouse brain section 72 hours after treatment. The blue staining is DAPI. MBS601235 was detected with streptavidin-Alexafluor 488 and the Nestin antibody was detected with a goat anti-mouse IgG-Alexafluor 594.
Mouse Anti-Neuron Specific Nuclear Protein Monoclonal Antibody, Testing Data – Cat# MBS601235

Immunohistochemistry (IHC) of anti-Neuron Specific Nuclear Protein antibody

MBS601235 staining pattern/morphology in rat cerebellum. Tissue pretreated with citrate, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select. Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Low magnification
Mouse Anti-Neuron Specific Nuclear Protein Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS601235

Testing Data of anti-Neuron Specific Nuclear Protein antibody

Mouse Anti-Neuron Specific Nuclear Protein Monoclonal Antibody, Testing Data – Cat# MBS601235

Other Notes

Small volumes of anti-Neuron Specific Nuclear Protein antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Neuron Specific Nuclear Protein purchase

MBS601235 is an antibody targeting Neuron Specific Nuclear Protein. Also known as Neuron Specific Nuclear Protein (NeuN) (Biotin). Reactive in Mouse, Rat. Host: Mouse.
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