Rat Anti-Rex-1 Monoclonal Antibody – Cat# MBS601296

Rex‑1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10ug/mL MBS601296 for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody and counterstained with DAPI (blue).

Rex-1 Antibody – Rat Monoclonal

Name: Rex-1 Antibody; Monoclonal; Rat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601296
Price: 1000 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601296

Human Rex-1 (also Zfp-42) is a 35kD zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc… More details

$1000 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Rex-1, Monoclonal Antibody

Full Product Name

Rex-1 (Reduced Expression Protein 1, Zinc Finger Protein 42, ZFP42)

Product Synonym Names

Anti -Rex-1 (Reduced Expression Protein 1, Zinc Finger Protein 42, ZFP42)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

7H157

Host

Rat

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Rex-1. Does not crossreact with recombinant human YY1.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Recombinant corresponding to aa1-150 of human Rex-1 expressed in E. coli (NP_777560).

Product Categories/Family for anti-Rex-1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

Applications Tested/Suitable for anti-Rex-1 antibody

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)

Application Notes for anti-Rex-1 antibody

Suitable for use in Immunocytochemistry, Western Blot and ELISA.
Dilution: Immunocytochemistry: 10ug/ml was used to detect REx-1 on NTERA2 cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted R1992-45 overnight at 4 degree C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane and under non-reducing and reducing conditions. ECL detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.

Other Notes

Small volumes of anti-Rex-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rex-1 purchase

MBS601296 is an antibody targeting Rex-1. Also known as Rex-1 (Reduced Expression Protein 1, Zinc Finger Protein 42, ZFP42). Reactive in Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Rex-1. It may not necessarily be applicable to this product.

NCBI GI #

209978599

NCBI Accession #

ACJ04679.1

NCBI Official Full Name

RNA exonuclease 1

Protein Family

Redox-sensing transcriptional repressor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.