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SET Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601341

SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with Sadenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure… More details

$465 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SET 7/9, Monoclonal Antibody
Full Product Name
SET 7/9
Product Synonym Names
Anti -SET 7/9
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4k15
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified from mouse ascitic fluid by Protein G affinity chromatography.
Specificity
Recognizes SET 7/9.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human SET 7/9
Hybridoma
Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SET7/9.

Product Categories/Family for anti-SET 7/9 antibody

Antibodies; Abs to Chromatin Proteins
Applications Tested/Suitable for anti-SET 7/9 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SET 7/9 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:250-1:2000; Recombinant SET7/9 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-SET7/9 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system.

Western Blot (WB) of anti-SET 7/9 antibody

Western Blot analysis of MBS601341. Recombinant SET7/9 was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS601341 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicated SET7/9(~50kD).
Mouse Anti-SET Monoclonal Antibody – Cat# MBS601341

Western Blot (WB) of anti-SET 7/9 antibody

Western Blot analysis of MBS601341. Cell lysates (5ug) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS601341 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system. |Lane 1.: 293T cell lysate|Lane 2.: SET7/9 transfected 293T cell lysate
Mouse Anti-SET Monoclonal Antibody, Western Blot (WB) – Cat# MBS601341

Immunocytochemistry (ICC) of anti-SET 7/9 antibody

Immunofluorescence/Immunocytochemistry analysis of SET7/9 in HeLa cells line using MBS601341 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) and |DAPI (Blue) for nucleus staining.
Mouse Anti-SET Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS601341

Other Notes

Small volumes of anti-SET 7/9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SET 7/9 purchase

MBS601341 is an antibody targeting SET 7/9. Reactive in Human. Host: Mouse.
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