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Thomsen-Friedenreich Antigen Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601388

The Thomsen-Fridenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy.2-4 It is considered as a pan-carcinoma marker. Can be applied for the… More details

$960 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thomsen-Friedenreich Antigen, Monoclonal Antibody
Full Product Name
Thomsen-Friedenreich Antigen
Product Synonym Names
Anti -Thomsen-Friedenreich Antigen
Clonality
Monoclonal
Isotype
IgM,k
Clone Number
0.N.567
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, with 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human and rat Thomsen-Friedenreich Antigen is specific for both anomeric forms of the disaccharide (TFa and TFb, including related structures on the glycolipid) and shows no crossreactivity with sialylated glycophorin.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Neuraminidase-treated human red blood cells. Epitope: Galb1-3GalNAc. Cellular Localization: Cell membrane.
Positive Control
KG1 cells and human colorectal carcinoma tissues.

Product Categories/Family for anti-Thomsen-Friedenreich Antigen antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-Thomsen-Friedenreich Antigen antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Thomsen-Friedenreich Antigen antibody
Suitable for use in Immunofluorescence and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT.
Staining formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0.

Immunohistochemistry (IHC) of anti-Thomsen-Friedenreich Antigen antibody

Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with NeoMarkers' Thomsen-Friedenreich Antigen antibody MBS601388 using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.
Mouse Anti-Thomsen-Friedenreich Antigen Monoclonal Antibody – Cat# MBS601388

Other Notes

Small volumes of anti-Thomsen-Friedenreich Antigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Thomsen-Friedenreich Antigen purchase

MBS601388 is an antibody targeting Thomsen-Friedenreich Antigen. Reactive in Human, Rat. Host: Mouse.
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