Mouse Anti-CLEC2D Monoclonal Antibody – Cat# MBS601570

Western blot analysis of OCIL in mouse spleen cell lysates. Thirty microgram of cell lysates per lane of mini gel was analyzed in Western blotting using IMG-387.

Osteoclast Inhibitory Lectin (CLEC2D) Antibody – Mouse Monoclonal

Name: CLEC2D Antibody; Monoclonal; Mouse; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601570
Price: 775 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601570

Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell… More details

$775 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Osteoclast Inhibitory Lectin (CLEC2D), Monoclonal Antibody

Full Product Name

Osteoclast Inhibitory Lectin (OCIL)

Product Gene Name

anti-CLEC2D antibody

Product Synonym Names

Anti -Osteoclast Inhibitory Lectin (OCIL)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

4H218

Host

Mouse

Species Reactivity

Mouse

Form/Format

Supplied as a liquid in PBS, 0.02% sodium azide.

Purity/Purification

Purified
Purified

Specificity

Recognizes mouse Osteoclast Inhibitory Lectin (OCIL)

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

21,849 Da

Chromosome Location

chromosome: 12; Location: 12p13

OMIM

605659

3D Structure

ModBase 3D Structure for Q9UHP7

Immunogen

Synthetic peptide containing amino acids 2-18 of mouse OCIL.

Product Categories/Family for anti-CLEC2D antibody

Antibodies; Abs to Lectins

Applications Tested/Suitable for anti-CLEC2D antibody

Western Blot (WB)

Application Notes for anti-CLEC2D antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1-2ug/ml Mouse spleen cell lysates can be used as positive control.

Other Notes

Small volumes of anti-CLEC2D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLEC2D purchase

MBS601570 is an antibody targeting CLEC2D. Also known as Osteoclast Inhibitory Lectin (OCIL). Reactive in Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CLEC2D. It may not necessarily be applicable to this product.

NCBI GI #

34369155

NCBI GeneID

29121

UniProt Primary Accession #

Q9UHP7

UniProt Secondary Accession #

Q6YID5; Q8WUP7; Q9HD37; Q9HD38; D6CI39; D6CI40; D6CI41

UniProt Related Accession #

Q9UHP7

NCBI Official Full Name

osteoclast inhibitory lectin

NCBI Official Synonym Full Names

C-type lectin domain family 2, member D

NCBI Official Symbol

CLEC2D

NCBI Official Synonym Symbols

CLAX; LLT1; OCIL; CLEC2D

UniProt Protein Name

C-type lectin domain family 2 member D

UniProt Synonym Protein Names

Lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; Osteoclast inhibitory lectin

Protein Family

C-type lectin domain family

UniProt Gene Name

CLEC2D

UniProt Synonym Gene Names

CLAX; LLT1; OCIL

UniProt Entry Name

CLC2D_HUMAN

NCBI Protein Information

C-type lectin domain family 2 member D; LLT-1; OTTHUMP00000205781; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, member D

NCBI Summary for CLEC2D

This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified, but the full-length nature of every transcript has not been defined. [provided by RefSeq]

UniProt Comments for CLEC2D

CLEC2D: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon- gamma. Binds high molecular weight sulfated glycosaminoglycans. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cell surface; membrane; integral to plasma membrane; endoplasmic reticulum

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction

Research Articles on CLEC2D

1. LLT1 used Src-PTK, p38 and ERK signalling pathways, but not PKC, PI3K or calcineurin pathways, to increase production of IFN-gamma by human natural killer cells.
2. Women with a lysine (GG genotype) at position 19 of the OCIL protein displayed lower bone mineral density at femoral neck and at lumbar spine sites than women having an asparagine residue.
3. Expression of LLT1 on activated dendritic cells and B cells suggests that it might regulate the cross-talk between NK cells and APCs
4. Observational study of gene-disease association. (HuGE Navigator)
5. Engagement of CD161 on NK cells with LLT1 expressed on target cells inhibited NK cell-mediated cytotoxicity and IFN-gamma secretion. LLT1/CD161 interaction in the presence of a TCR signal enhanced IFN-gamma production by T cells
6. LLT1 induces Interferon Type II production by natural killer cells.
7. LLT1 on target cells can inhibit NK cytotoxicity via interactions with CD161. LLT1 activates NFAT-GFP reporter cells expressing a CD3zeta-CD161 chimeric receptor; reciprocally, reporter cells with a CD3zeta-LLT1 chimeric receptor are stimulated by CD161
8. Data show that osteoclast inhibitory lectin (OCIL) binds a range of physiologically important glycosaminoglycans, and this property may modulate OCIL actions upon other cells [OCIL].

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CLEC2D antibody

Reference Product	PubMed Publications
KLRB1 antibody	>10 publications with CLEC2D and KLRB1

Diseases associated with anti-CLEC2D antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with CLEC2D and Neoplasms

Organs/Tissues associated with anti-CLEC2D antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with CLEC2D and Blood
Skin Antibodies	>2 publications with CLEC2D and Skin
Bone Marrow Antibodies	>2 publications with CLEC2D and Bone Marrow
Uterus Antibodies	>1 publications with CLEC2D and Uterus
Brain Antibodies	>1 publications with CLEC2D and Brain
Placenta Antibodies	>1 publications with CLEC2D and Placenta