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GATAD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601575

MBS601575 is an antibody targeting GATAD1. Also known as GATAD1 (GATA Zinc Finger Domain Containing 1, ODAG, FLJ22489, RG083M05.2). Reactive in Human, Mouse. Host: Mouse. More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GATAD1, Monoclonal Antibody
Full Product Name
GATAD1 (GATA Zinc Finger Domain Containing 1, ODAG, FLJ22489, RG083M05.2)
Product Gene Name
anti-GATAD1 antibody
Product Synonym Names
Anti -GATAD1 (GATA Zinc Finger Domain Containing 1, ODAG, FLJ22489, RG083M05.2)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8C155
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human GATAD1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
28,690 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (92076765..92088742). Location: 7q21-q22
3D Structure
ModBase 3D Structure for Q8WUU5
Immunogen
Partial sequence of recombinant full-length protein to human GATAD1

Product Categories/Family for anti-GATAD1 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-GATAD1 antibody
Western Blot (WB)
Application Notes for anti-GATAD1 antibody
Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000

Testing Data of anti-GATAD1 antibody

Mouse Anti-GATAD1 Monoclonal Antibody – Cat# MBS601575

Western Blot (WB) of anti-GATAD1 antibody

Detection of GATAD1 by Western blot. Samples: Whole cell lysate from human A2058 (H, 50 μg) and mouse NIH3T3 (M, 50 μg) cells. Predicted molecular weight: 28 kD
Mouse Anti-GATAD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS601575

Testing Data of anti-GATAD1 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS601575 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.
Mouse Anti-GATAD1 Monoclonal Antibody, Testing Data – Cat# MBS601575

Immunostaining (IS) of anti-GATAD1 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS601575 mAb.
Mouse Anti-GATAD1 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS601575

Other Notes

Small volumes of anti-GATAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GATAD1. It may not necessarily be applicable to this product.

NCBI GI #
88759346
NCBI GeneID
57798
NCBI Accession #
NP_066990.3
UniProt Primary Accession #
Q8WUU5
UniProt Secondary Accession #
Q8N5Y5; Q99995; Q9H689; B2RE37; D6W5Q5
UniProt Related Accession #
Q8WUU5
GenBank Nucleotide #
NM_021167.3
NCBI Official Full Name
GATA zinc finger domain-containing protein 1
NCBI Official Synonym Full Names
GATA zinc finger domain containing 1
NCBI Official Symbol
GATAD1
NCBI Official Synonym Symbols
ODAG; FLJ22489; FLJ40695; RG083M05.2
UniProt Protein Name
GATA zinc finger domain-containing protein 1
UniProt Synonym Protein Names
Ocular development-associated gene protein
Protein Family
GATA zinc finger domain-containing protein
UniProt Gene Name
GATAD1
UniProt Synonym Gene Names
ODAG
UniProt Entry Name
GATD1_HUMAN
NCBI Protein Information
GATA zinc finger domain-containing protein 1; OTTHUMP00000161848; ocular development associated; ocular development-associated gene protein

NCBI Summary for GATAD1

The protein encoded by this gene contains a zinc finger at the N-terminus, and is thought to bind to a histone modification site that regulates gene expression. Mutations in this gene have been associated with autosomal recessive dilated cardiomyopathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]
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UniProt Comments for GATAD1

GATAD1: Component of some chromatin complex recruited to chromatin sites methylated 'Lys-4' of histone H3 (H3K4me). Defects in GATAD1 are the cause of cardiomyopathy, dilated type 2B (CMD2B). CMD2B is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent

Disease: Cardiomyopathy, Dilated, 2b
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Research Articles on GATAD1

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-GATAD1 antibody

 Disease Name  Pubmed Publications
 Stroke Antibodies  >1 publications with GATAD1 and Stroke

Organs/Tissues associated with anti-GATAD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >2 publications with GATAD1 and Eye
 Heart Antibodies  >1 publications with GATAD1 and Heart
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