• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Threonine-Proline Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601652

The MAPK and CDK family of serine/ threonine protein kinases plays important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK… More details

$830 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Threonine-Proline, Monoclonal Antibody
Full Product Name
Threonine-Proline (Thr-Pro), phosphorylated
Product Synonym Names
Anti -Threonine-Proline (Thr-Pro), phosphorylated
Clonality
Monoclonal
Isotype
IgM
Clone Number
3A40 (P-Thr-Pro-101)
Host
Mouse
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes Mouse phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1).
Cross Reactivity: All Species
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphothreonine- proline-containing peptides. Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

Product Categories/Family for anti-Threonine-Proline antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-Threonine-Proline antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Threonine-Proline antibody
WB: 1:5000
IHC-P: 1:400
ELISA(peptide): 1:1000
Other applications not yet tested
Optimal dilutions/concentrations should be determined by the end user.
For western blots, incubate membrane with diluted antibody, 5% BSA, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-Threonine-Proline antibody

Western blot analysis of whole cell lysates from Jurkat cells untreated and treated with 1 ug/ml nocadazole for 12 hours prior to lysis, using Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101). Proteins were separated by 2D electrophoresis prior to blotting.
Mouse Anti-Threonine-Proline Monoclonal Antibody – Cat# MBS601652

Western Blot (WB) of anti-Threonine-Proline antibody

Western blot analysis of extracts from serum and okadaic acid treated COS cells, using Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) (left) and total protein staining (right).
Mouse Anti-Threonine-Proline Monoclonal Antibody, Western Blot (WB) – Cat# MBS601652

Immunohistochemistry (IHC) of anti-Threonine-Proline antibody

Immunohistochemical staining of proteins containing phospho-threonine-proline motifs in paraffin-embedded human breast carcinoma, using Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101).
Mouse Anti-Threonine-Proline Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS601652

Other Notes

Small volumes of anti-Threonine-Proline antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Threonine-Proline purchase

MBS601652 is an antibody targeting Threonine-Proline. Also known as Threonine-Proline (Thr-Pro), phosphorylated. Host: Mouse.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000