• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MGMT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601697

O6-Methylguanine-DNA Methyltransferase (MGMT) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine, primarily O6 methylguanine lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and… More details

$1015 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MGMT, Monoclonal Antibody
Full Product Name
MGMT (O6-Methylguanine DNA Methyltransferase)
Product Gene Name
anti-Mgmt antibody
Product Synonym Names
Anti -MGMT (O6-Methylguanine DNA Methyltransferase)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q434
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography
Specificity
Recognizes MGMT in human tissues, tumors, cells or xenograft lines at ~22-25kD. Gives weak cross-reactivity with MGMT from mouse, hampster and rat cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,244 Da
Chromosome Location
Chromosome: 1; NC_005100.3 (216161435..216388310). Location: 1q41
3D Structure
ModBase 3D Structure for P24528
Immunogen
Recombinant protein corresponding to human MGMT (O6-methylguanine-DNA methyltransferase) expressed in E. coli.
Positive Control
Tonsil tissue, HeLa cells, CEM-CCRF cells.

Product Categories/Family for anti-Mgmt antibody

Antibodies; Abs to Enzymes; Methyltransferase
Applications Tested/Suitable for anti-Mgmt antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Mgmt antibody
Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence.
Dilution: Western Blot: 1:500 detects MGMT on 10ug of Jurkat lysates.
Immunohistochemistry: Paraffin sections
Immunofluorescence (IC): HeLa cells

Western Blot (WB) of anti-Mgmt antibody

Western Blot Analysis: Representative lot data.|HEK293 lysate (Lane 1), HeLa lysate (Lane 2), HepG2 lysate (Lane 3), Jurkat lysate (Lane 4) and PC3 lysate (Lane 5) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS601697. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein MGMT (~22kD).
Mouse Anti-MGMT Monoclonal Antibody – Cat# MBS601697

Western Blot (WB) of anti-Mgmt antibody

Western Blot: Representative lot data. Jurkat lysate resolved by electrophoresis, transferred to PVDF membrane & probed with MBS601697 Proteins were visualized using goat anti-mouse secondary Ab conjugated to HRP chemiluminescence detection system.|Arrow indicates MGMT (22kD).
Mouse Anti-MGMT Monoclonal Antibody, Western Blot (WB) – Cat# MBS601697

Immunohistochemistry (IHC) of anti-Mgmt antibody

Immunohistochemistry staining of MGMT in human breast cancer using MBS601697.
Mouse Anti-MGMT Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS601697

Immunofluorescence (IF) of anti-Mgmt antibody

Confolcal fluorescent analysis of HeLa using MBS601697 (Red). Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.
Mouse Anti-MGMT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS601697

Other Notes

Small volumes of anti-Mgmt antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mgmt purchase

MBS601697 is an antibody targeting Mgmt. Also known as MGMT (O6-Methylguanine DNA Methyltransferase). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Mgmt. It may not necessarily be applicable to this product.

NCBI GI #
127071
NCBI GeneID
25332
NCBI Accession #
P24528.3
UniProt Primary Accession #
P24528
UniProt Related Accession #
P24528
NCBI Official Full Name
Methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
Mgmt
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names
6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Protein Family
Probable methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name
Mgmt
UniProt Synonym Gene Names
MGMT
UniProt Entry Name
MGMT_RAT
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase; O6-alkylguanine-DNA alkyltransferase; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA-alkyltransferase; 0-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase

NCBI Summary for Mgmt

enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]

UniProt Comments for Mgmt

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; EC 2.1.1.63; Methyltransferase

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding

Biological Process: methylation; response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA repair; regulation of caspase activity; response to organic cyclic substance; response to DNA damage stimulus
Show more

Research Articles on Mgmt

1. Folate deficiency resulted in significant increases in levels of the DNA repair proteins OGG-1 and MGMT in the liver

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Mgmt antibody

 Reference Product  PubMed Publications
 Mlh1 antibody  >197 publications with Mgmt and Mlh1
 Tp53 antibody  >93 publications with Mgmt and Tp53
 Pten antibody  >72 publications with Mgmt and Pten
 Apex1 antibody  >12 publications with Mgmt and Apex1
 Nthl1 antibody  >1 publications with Mgmt and Nthl1

Pathways associated with anti-Mgmt antibody

 Products by Pathway  Pathway Diagram
 DNA Damage Reversal Pathway antibodies  DNA Damage Reversal Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram

Diseases associated with anti-Mgmt antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1732 publications with Mgmt and Neoplasms
 Glioma Antibodies  >776 publications with Mgmt and Glioma
 Nervous System Diseases Antibodies  >681 publications with Mgmt and Nervous System Diseases
 Brain Neoplasms Antibodies  >664 publications with Mgmt and Brain Neoplasms
 Glioblastoma Antibodies  >553 publications with Mgmt and Glioblastoma
 Carcinoma Antibodies  >414 publications with Mgmt and Carcinoma
 Recurrence Antibodies  >181 publications with Mgmt and Recurrence
 Adenocarcinoma Antibodies  >153 publications with Mgmt and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >129 publications with Mgmt and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >121 publications with Mgmt and Lung Neoplasms

Organs/Tissues associated with anti-Mgmt antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >712 publications with Mgmt and Brain
 Prostate Antibodies  >26 publications with Mgmt and Prostate
 Ovary Antibodies  >20 publications with Mgmt and Ovary
 Heart Antibodies  >5 publications with Mgmt and Heart
 Connective Tissue Antibodies  >1 publications with Mgmt and Connective Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000