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Neurofilament M 145-160kD (NEFM) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601733

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at… More details

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Product Overview

Online Datasheet in PDF
Product Name
Neurofilament M 145-160kD (Nefm), Monoclonal Antibody
Full Product Name
Neurofilament M 145-160kD (NF-M)
Product Gene Name
anti-Nefm antibody
Product Synonym Names
Anti -Neurofilament M 145-160kD (NF-M)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6H9
Host
Mouse
Species Reactivity
Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity/Purification
Supernatant
Tissue culture supernatant
Specificity
The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. Recognizes intact NF-M strongly and specifically on Western Blots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
95,916 Da
Chromosome Location
Chromosome: 14; NC_000080.5 (68737602..68743061, complement). Location: 14 D1; 14
3D Structure
ModBase 3D Structure for P08553
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli (1).

Product Categories/Family for anti-Nefm antibody

Antibodies; Abs to Neurofilament
Applications Tested/Suitable for anti-Nefm antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-Nefm antibody
Suitable for use in Immunofluorescence, Western Blotting.
Dilution: Immunofluorescence: 1:100
Western Blotting: 1:1000

Other Notes

Small volumes of anti-Nefm antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nefm purchase

MBS601733 is an antibody targeting Nefm. Also known as Neurofilament M 145-160kD (NF-M). Reactive in Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Nefm. It may not necessarily be applicable to this product.

NCBI GI #
112363107
NCBI GeneID
18040
NCBI Accession #
NP_032717.2
UniProt Primary Accession #
P08553
UniProt Secondary Accession #
Q0VDM8; Q3HRJ6; Q3TNS4; Q3TPK2; Q61961; Q8BQ20; A2VCT5
UniProt Related Accession #
P08553
GenBank Nucleotide #
NM_008691.2
NCBI Official Full Name
neurofilament medium polypeptide
NCBI Official Synonym Full Names
neurofilament, medium polypeptide
NCBI Official Symbol
Nefm
NCBI Official Synonym Symbols
Nfm; NF-M; Nef3; NF160; NF165
UniProt Protein Name
Neurofilament medium polypeptide
UniProt Synonym Protein Names
160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
Protein Family
Neurofilament medium polypeptide
UniProt Gene Name
Nefm
UniProt Synonym Gene Names
Nef3; Nfm
UniProt Entry Name
NFM_MOUSE
NCBI Protein Information
neurofilament medium polypeptide; neurofilament-M; OTTMUSP00000017611; neurofilament 3, medium; neurofilament protein M; 160 kDa neurofilament protein; neurofilament triplet M protein

UniProt Comments for Nefm

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Cellular Component: neuron projection; cytoskeleton; axon; cell; neurofilament; intermediate filament; intracellular; neuromuscular junction; myelin sheath

Molecular Function: structural molecule activity

Biological Process: neurofilament cytoskeleton organization and biogenesis; regulation of axon diameter; axon cargo transport; intermediate filament cytoskeleton organization and biogenesis; intermediate filament bundle assembly; microtubule cytoskeleton organization and biogenesis
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Research Articles on Nefm

1. results obtained in mouse model for leprous neuropathy indicate biochemical alterations in neurofilaments, i.e., hyperphosphorylation of NF-H and NF-M

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Nefm antibody

 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with anti-Nefm antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with Nefm and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with Nefm and Nervous System Diseases
 Nervous System Malformations Antibodies  >1 publications with Nefm and Nervous System Malformations
 Brain Neoplasms Antibodies  >1 publications with Nefm and Brain Neoplasms
 Hereditary Sensory and Motor Neuropathy Antibodies  >1 publications with Nefm and Hereditary Sensory and Motor Neuropathy
 Nervous System Neoplasms Antibodies  >1 publications with Nefm and Nervous System Neoplasms
 Polyneuropathies Antibodies  >1 publications with Nefm and Polyneuropathies
 Peripheral Nervous System Diseases Antibodies  >1 publications with Nefm and Peripheral Nervous System Diseases

Organs/Tissues associated with anti-Nefm antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with Nefm and Brain
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