Mouse Anti-WISP1 Monoclonal Antibody – Cat# MBS601770

WIF‑1 in Human Breast. WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8ug/mL MBS601770

Wnt Inhibiting Factor 1 (WISP1) Antibody – Mouse Monoclonal

Name: WISP1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601770
Price: 755 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601770

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. More details

$755 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Wnt Inhibiting Factor 1 (Wisp1), Monoclonal Antibody

Full Product Name

Wnt Inhibiting Factor 1 (WIF-1)

Product Gene Name

anti-Wisp1 antibody

Product Synonym Names

Anti -Wnt Inhibiting Factor 1 (WIF-1)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

8H102

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 100ul sterile PBS.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Wnt Inhibiting Factor 1. Shows less than 5% crossreactivity with recombinant mouse WIF-1.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

40,703 Da

Chromosome Location

Chromosome: 15; NC_000081.6 (66891393..66923199). Location: 15 D2; 15 29.3 cM

3D Structure

ModBase 3D Structure for O54775

Immunogen

Recombinant protein corresponding to aa29-379 from human WIF-1, expressed in NSO cells. (Accession #AAD25402).

Hybridoma

Mouse myeloma with B cells from a mouse immunized with purified, NS0-derived, recombinant human WIF-1.

Product Categories/Family for anti-Wisp1 antibody

Antibodies; Abs to Binding Proteins

Applications Tested/Suitable for anti-Wisp1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-Wisp1 antibody

Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry (Paraffin): 8-25ug/ml; Used to detect WIF-1 in immersion fixed paraffin-embedded human breast sections.

Other Notes

Small volumes of anti-Wisp1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Wisp1 purchase

MBS601770 is an antibody targeting Wisp1. Also known as Wnt Inhibiting Factor 1 (WIF-1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Wisp1. It may not necessarily be applicable to this product.

NCBI GI #

9256533

NCBI GeneID

22402

NCBI Accession #

NP_061353.1

UniProt Primary Accession #

O54775

UniProt Secondary Accession #

Q80ZL1

UniProt Related Accession #

O54775

GenBank Nucleotide #

NM_018865.2

NCBI Official Full Name

WNT1-inducible-signaling pathway protein 1

NCBI Official Synonym Full Names

WNT1 inducible signaling pathway protein 1

NCBI Official Symbol

Wisp1

NCBI Official Synonym Symbols

CCN4; Elm1; AW146261

UniProt Protein Name

WNT1-inducible-signaling pathway protein 1

UniProt Synonym Protein Names

CCN family member 4; ELM-1

Protein Family

WNT1-inducible-signaling pathway protein

UniProt Gene Name

Wisp1

UniProt Synonym Gene Names

Ccn4; Elm1; WISP-1

UniProt Entry Name

WISP1_MOUSE

NCBI Protein Information

WNT1-inducible-signaling pathway protein 1; ELM-1; WISP-1; CCN family member 4

UniProt Comments for Wisp1

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Oncoprotein; Secreted

Cellular Component: proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding

Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction

Research Articles on Wisp1

1. WISP-1 exerts paracrine action on immune cells by inhibiting their response to IL12

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-Wisp1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>48 publications with Wisp1 and Neoplasms
Carcinoma Antibodies	>17 publications with Wisp1 and Carcinoma
Inflammation Antibodies	>11 publications with Wisp1 and Inflammation
Colorectal Neoplasms Antibodies	>10 publications with Wisp1 and Colorectal Neoplasms
Breast Neoplasms Antibodies	>9 publications with Wisp1 and Breast Neoplasms
Fibrosis Antibodies	>9 publications with Wisp1 and Fibrosis
Adenocarcinoma Antibodies	>8 publications with Wisp1 and Adenocarcinoma
Cardiovascular Diseases Antibodies	>8 publications with Wisp1 and Cardiovascular Diseases
Cell Transformation, Neoplastic Antibodies	>7 publications with Wisp1 and Cell Transformation, Neoplastic
Neoplasm Metastasis Antibodies	>6 publications with Wisp1 and Neoplasm Metastasis

Organs/Tissues associated with anti-Wisp1 antibody

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>31 publications with Wisp1 and Connective Tissue
Bone Antibodies	>21 publications with Wisp1 and Bone
Lung Antibodies	>14 publications with Wisp1 and Lung
Embryonic Tissue Antibodies	>11 publications with Wisp1 and Embryonic Tissue
Muscle Antibodies	>9 publications with Wisp1 and Muscle
Bone Marrow Antibodies	>7 publications with Wisp1 and Bone Marrow
Skin Antibodies	>7 publications with Wisp1 and Skin
Brain Antibodies	>6 publications with Wisp1 and Brain
Heart Antibodies	>5 publications with Wisp1 and Heart
Joint Antibodies	>4 publications with Wisp1 and Joint