• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ROCK-1 (rok) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601792

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of GTPase Rho and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified (ROCK1 and ROCK2). ROCK is composed of… More details

$565 / 0.02 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ROCK-1 (rok), Monoclonal Antibody
Full Product Name
ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)
Product Gene Name
anti-rok antibody
Product Synonym Names
Anti -ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)
Clonality
Monoclonal
Isotype
IgG
Clone Number
8A34
Host
Rabbit
Species Reactivity
Mouse, rat, monkey
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified
Specificity
Recognizes endogenous levels of total human ROCK1.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
160,307 Da
Chromosome Location
Chromosome: X; NC_004354.3 (16519142..16531452, complement). Location: 14F2-14F4
3D Structure
ModBase 3D Structure for Q9VXE3
Grade
Purified
Immunogen
Synthetic peptide corresponding to the central sequence of human ROCK1

Product Categories/Family for anti-rok antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-rok antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-rok antibody
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-rok antibody

Western bBot analysis of extracts from various cell types using MBS601792.
Rabbit Anti-rok Monoclonal Antibody – Cat# MBS601792

Western Blot (WB) of anti-rok antibody

Immunoprecipitation of ROCK1 from HeLa cell extracts using MBS601792. Western blot detection was perfomed using the same antibody, MBS601792. Lane 1 is 5% input.
Rabbit Anti-rok Monoclonal Antibody, Western Blot (WB) – Cat# MBS601792

Other Notes

Small volumes of anti-rok antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rok purchase

MBS601792 is an antibody targeting rok. Also known as ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI). Reactive in Mouse, rat, monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for rok. It may not necessarily be applicable to this product.

NCBI GI #
24642569
NCBI GeneID
43916
NCBI Accession #
NP_536796.2
UniProt Primary Accession #
Q9VXE3
UniProt Related Accession #
Q9VXE3
GenBank Nucleotide #
NM_080535.2
NCBI Official Full Name
Rho-kinase
NCBI Official Synonym Full Names
Rho-kinase
NCBI Official Symbol
rok
NCBI Official Synonym Symbols
CG9774; DmelCG9774; DRhk; drok; dRok; dROK; Drok; DRok; DROK; Rhk; RhoK; Rock; ROCK; ROCK-1; ROCK1; Rok; ROK; ROKalpha/beta
UniProt Protein Name
FI04037p
Protein Family
Represso
UniProt Gene Name
rok
UniProt Synonym Gene Names
rok-RA
UniProt Entry Name
Q9VXE3_DROME
NCBI Protein Information
CG9774 gene product from transcript CG9774-RA; CG9774-PA; DRho-kinase; ROCK; Rho-associated kinase; RhoA kinase; drosophila rho-associated kinase; rho kinase; rok-PA

UniProt Comments for rok

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. SAAS SAAS002219

Sequence similarities: Contains AGC-kinase C-terminal domain. SAAS SAAS002219Contains PH domain. SAAS SAAS002219Contains protein kinase domain. SAAS SAAS002219

Research Articles on rok

1. Atonal and EGFR signalling orchestrate rok- and Drak-dependent adherens junction remodelling during ommatidia morphogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-rok antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 G Alpha (12/13) Signalling Events Pathway antibodies  G Alpha (12/13) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Sema4D In Semaphorin Signaling Pathway antibodies  Sema4D In Semaphorin Signaling Pathway Diagram
 Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies  Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram

Organs/Tissues associated with anti-rok antibody

 Organ/Tissue Name  Pubmed Publications
 Whole Body Antibodies  >1 publications with rok and Whole Body
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000