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Histidyl-Trna Synthetase (hisS) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601817

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acid. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the… More details

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Product Overview

Online Datasheet in PDF
Product Name
Histidyl-tRNA Synthetase (hisS), Monoclonal Antibody
Full Product Name
Histidyl-tRNA Synthetase (HRS, HARS, FLJ20491)
Product Gene Name
anti-hisS antibody
Product Synonym Names
Anti -Histidyl-tRNA Synthetase (HRS, HARS, FLJ20491)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C179
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Histidyl-tRNA Synthetase. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,029 Da
Chromosome Location
NC_000913.2 (2637323..2638597, complement)
3D Structure
ModBase 3D Structure for P60906
Immunogen
Synthetic peptide from the N-terminal region of human Histidyl-tRNA Synthetase

Product Categories/Family for anti-hisS antibody

Antibodies; Abs to Enzymes; Synthetase
Applications Tested/Suitable for anti-hisS antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-hisS antibody
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Flow Cytometry: 0.5-2ug/sample
Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug/sample
Immunocytochemistry: 2-100ug/ml

Western Blot (WB) of anti-hisS antibody

Detection of HARS by Western blot. Samples usinf MBS601817: Whole cell lysate from human HeLa (25ug) , mouse NIH3T3 (25ug) and rat F2408 (25ug) cells. Predicted molecular weight: 57kD
Mouse Anti-hisS Monoclonal Antibody – Cat# MBS601817

Immunoprecipitation (IP) of anti-hisS antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS601817.
Mouse Anti-hisS Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS601817

Testing Data of anti-hisS antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS601817 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.
Mouse Anti-hisS Monoclonal Antibody, Testing Data – Cat# MBS601817

Immunostaining (IS) of anti-hisS antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS601817.
Mouse Anti-hisS Monoclonal Antibody, Immunostaining (IS) – Cat# MBS601817

Testing Data of anti-hisS antibody

Mouse Anti-hisS Monoclonal Antibody, Testing Data – Cat# MBS601817

Other Notes

Small volumes of anti-hisS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for hisS purchase

MBS601817 is an antibody targeting hisS. Also known as Histidyl-tRNA Synthetase (HRS, HARS, FLJ20491). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for hisS. It may not necessarily be applicable to this product.

NCBI GI #
16130439
NCBI GeneID
946989
NCBI Accession #
NP_417009.1
UniProt Primary Accession #
P60906
UniProt Secondary Accession #
P04804
GenBank Nucleotide #
NC_000913.2
NCBI Official Full Name
histidyl tRNA synthetase
NCBI Official Symbol
hisS
NCBI Official Synonym Symbols
ECK2510; JW2498
UniProt Protein Name
Histidyl-tRNA synthetase
UniProt Synonym Protein Names
Histidine--tRNA ligase
Protein Family
Histidine--tRNA ligase
UniProt Gene Name
hisS
UniProt Entry Name
SYH_ECOLI
NCBI Protein Information
histidyl tRNA synthetase

NCBI Summary for hisS

Histidyl-tRNA synthetase (HisRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10453].

UniProt Comments for hisS

Catalytic activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127

Subunit structure: Homodimer.

Subcellular location: Cytoplasm HAMAP MF_00127.

Sequence similarities: Belongs to the class-II aminoacyl-tRNA synthetase family.

Research Articles on hisS

1. N-terminus verified by Edman degradation on mature peptide

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-hisS antibody

 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
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