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Nitrogen Fixation 1 Homolog (NFS1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601824

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame… More details

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Product Overview

Online Datasheet in PDF
Product Name
NFS1 Nitrogen Fixation 1 Homolog, Monoclonal Antibody
Full Product Name
NFS1 Nitrogen Fixation 1 Homolog (HUSSY-08, IscS, NFS1, NIFS)
Product Gene Name
anti-NFS1 antibody
Product Synonym Names
Anti -NFS1 Nitrogen Fixation 1 Homolog (HUSSY-08, IscS, NFS1, NIFS)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C236
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human NFS1 Nitrogen Fixation 1 Homolog. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
50,186 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (34256611..34287274, complement). Location: 20q11.22
3D Structure
ModBase 3D Structure for Q53FP3
Immunogen
Partial sequence of recombinant full-length protein to human NFS1 Nitrogen Fixation 1 Homolog

Product Categories/Family for anti-NFS1 antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-NFS1 antibody
Western Blot (WB)
Application Notes for anti-NFS1 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-NFS1 antibody

Western blot analysis of immunized recombinant protein, using MBS601824.
Mouse Anti-NFS1 Monoclonal Antibody – Cat# MBS601824

Western Blot (WB) of anti-NFS1 antibody

Detection of NFS1 by Western Blot using MBS601824. Samples: Whole cell lysate from human HEK293 (H, 25ug), mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 50kD.
Mouse Anti-NFS1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS601824

Other Notes

Small volumes of anti-NFS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFS1 purchase

MBS601824 is an antibody targeting NFS1. Also known as NFS1 Nitrogen Fixation 1 Homolog (HUSSY-08, IscS, NFS1, NIFS). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NFS1. It may not necessarily be applicable to this product.

NCBI GI #
55958847
NCBI GeneID
9054
UniProt Primary Accession #
Q53FP3
UniProt Related Accession #
Q53FP3; Q5QP19; Q8WV90; Q9Y697
NCBI Official Full Name
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
NCBI Official Synonym Full Names
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
NCBI Official Symbol
NFS1
NCBI Official Synonym Symbols
IscS; NIFS; HUSSY-08
UniProt Protein Name
NFS1 nitrogen fixation 1 isoform a variant
Protein Family
Cysteine desulfurase
UniProt Entry Name
Q53FP3_HUMAN
NCBI Protein Information
cysteine desulfurase, mitochondrial; OTTHUMP00000030799; OTTHUMP00000030800; OTTHUMP00000179026; nitrogen-fixing bacteria S-like protein; nitrogen fixation 1 (S. cerevisiae, homolog)

NCBI Summary for NFS1

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq]
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UniProt Comments for NFS1

NFS1: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Transferase; Cofactor and Vitamin Metabolism - thiamine; Mitochondrial; EC 2.8.1.7

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; cysteine desulfurase activity; pyridoxal phosphate binding

Biological Process: iron incorporation into metallo-sulfur cluster; cysteine metabolic process; vitamin metabolic process; sulfur amino acid metabolic process; protein complex assembly; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process
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Research Articles on NFS1

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NFS1 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 Molybdenum Cofactor Biosynthesis Pathway antibodies  Molybdenum Cofactor Biosynthesis Pathway Diagram
 Sulfur Relay System Pathway antibodies  Sulfur Relay System Pathway Diagram
 Sulfur Relay System Pathway antibodies  Sulfur Relay System Pathway Diagram
 Thiamine Metabolism Pathway antibodies  Thiamine Metabolism Pathway Diagram
 Thiamine Metabolism Pathway antibodies  Thiamine Metabolism Pathway Diagram

Diseases associated with anti-NFS1 antibody

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >4 publications with NFS1 and Neurodegenerative Diseases
 Pain Antibodies  >2 publications with NFS1 and Pain

Organs/Tissues associated with anti-NFS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with NFS1 and Liver
 Muscle Antibodies  >1 publications with NFS1 and Muscle
 Spleen Antibodies  >1 publications with NFS1 and Spleen
 Blood Antibodies  >1 publications with NFS1 and Blood
 Heart Antibodies  >1 publications with NFS1 and Heart
 Kidney Antibodies  >1 publications with NFS1 and Kidney
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