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CTF18, Chromosome Transmission Fidelity Factor 18 (CHTF18) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601947

MBS601947 is an antibody targeting CHTF18. Also known as CTF18, Chromosome Transmission Fidelity Factor 18 (CHTF18, CHL12, C16orf41, RUVBL). Reactive in Human. Host: Mouse. More details

$685 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CTF18, Chromosome Transmission Fidelity Factor 18 (CHTF18), Monoclonal Antibody
Full Product Name
CTF18, Chromosome Transmission Fidelity Factor 18 (CHTF18, CHL12, C16orf41, RUVBL)
Product Gene Name
anti-CHTF18 antibody
Product Synonym Names
Anti -CTF18, Chromosome Transmission Fidelity Factor 18 (CHTF18, CHL12, C16orf41, RUVBL)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C89
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CTF18, Chromosome Transmission Fidelity Factor 18.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
107,383 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (838622..848074). Location: 16p13.3
OMIM
613201
3D Structure
ModBase 3D Structure for Q8WVB6
Immunogen
Partial sequence of recombinant full-length protein to human CTF18, Chromosome Transmission Fidelity Factor 18

Product Categories/Family for anti-CHTF18 antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-CHTF18 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CHTF18 antibody
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1-2ug/ml or 1:500-1:1000
Immunoprecipitation: 1-2ug/mg of total cell protein (~10e7 cells)

Testing Data of anti-CHTF18 antibody

Mouse Anti-CHTF18 Monoclonal Antibody – Cat# MBS601947

Western Blot (WB) of anti-CHTF18 antibody

Detection of MBS601947 by Western blot. Samples: Whole cell lysate (25 μg) from A2058 cells. Predicted molecular weight: 107 kD
Mouse Anti-CHTF18 Monoclonal Antibody, Western Blot (WB) – Cat# MBS601947

Immunoprecipitation (IP) of anti-CHTF18 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS601947.
Mouse Anti-CHTF18 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS601947

Other Notes

Small volumes of anti-CHTF18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHTF18. It may not necessarily be applicable to this product.

NCBI GI #
27501458
NCBI GeneID
63922
NCBI Accession #
NP_071375.1
UniProt Primary Accession #
Q8WVB6
UniProt Secondary Accession #
Q7Z6Y4; Q7Z6Y6; Q9BR83; Q9BRG5; Q9H7K3; B7ZBA2; D3DU68
UniProt Related Accession #
Q8WVB6; Q96S08
GenBank Nucleotide #
NM_022092.2
NCBI Official Full Name
chromosome transmission fidelity protein 18 homolog
NCBI Official Synonym Full Names
CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
NCBI Official Symbol
CHTF18
NCBI Official Synonym Symbols
CHL12; Ctf18; RUVBL; C16orf41; C321D2.2; C321D2.3; C321D2.4
UniProt Protein Name
Chromosome transmission fidelity protein 18 homolog
UniProt Synonym Protein Names
CHL12
Protein Family
Chromosome transmission fidelity protein
UniProt Gene Name
CHTF18
UniProt Synonym Gene Names
C16orf41; CTF18
UniProt Entry Name
CTF18_HUMAN
NCBI Protein Information
chromosome transmission fidelity protein 18 homolog; hCTF18; OTTHUMP00000045506; OTTHUMP00000208409; homolog of yeast CHL12; C321D2.3 (novel protein); C321D2.4 (novel protein); some homology with holliday junction DNA helicase RUVB like

NCBI Summary for CHTF18

CHTF18, CHTF8 (MIM 613202), and DCC1 (DSCC1; MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176]; Bermudez et al., 2003 [PubMed 12930902]).[supplied by OMIM]
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UniProt Comments for CHTF18

CTF18: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single- stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18- RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH. Belongs to the activator 1 small subunits family. CTF18 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; membrane; cytoplasm

Molecular Function: DNA binding; ATP binding

Biological Process: negative regulation of chromosome segregation; meiotic recombination; spermatogenesis; DNA replication; male meiosis
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Research Articles on CHTF18

1. Stable interaction between the human proliferating cell nuclear antigen loader complex Ctf18-replication factor C (RFC) and DNA polymerase {epsilon} is mediated by the cohesion-specific subunits, Ctf18, Dcc1, and Ctf8.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHTF18 antibody

 Reference Product  PubMed Publications
 PCNA antibody  >7 publications with CHTF18 and PCNA

Diseases associated with anti-CHTF18 antibody

 Disease Name  Pubmed Publications
 Drug Toxicity Antibodies  >1 publications with CHTF18 and Drug Toxicity

Organs/Tissues associated with anti-CHTF18 antibody

 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >1 publications with CHTF18 and Ovary
 Testis Antibodies  >1 publications with CHTF18 and Testis
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