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DHX38 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602008

MBS602008 is an antibody targeting DHX38. Also known as DHX38 (DEAH (Asp-Glu-Ala-His) Box 38, DDX38, KIAA0224, PRPF16). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DHX38, Monoclonal Antibody
Full Product Name
DHX38 (DEAH (Asp-Glu-Ala-His) Box 38, DDX38, KIAA0224, PRPF16)
Product Gene Name
anti-DHX38 antibody
Product Synonym Names
Anti -DHX38 (DEAH (Asp-Glu-Ala-His) Box 38, DDX38, KIAA0224, PRPF16)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C117
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human DHX38. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
140,503 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (72127615..72146811). Location: 16q22
OMIM
605584
3D Structure
ModBase 3D Structure for Q92620
Immunogen
Partial sequence of recombinant full-length protein to human DHX38

Product Categories/Family for anti-DHX38 antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-DHX38 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-DHX38 antibody
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2-50ug/ml

Testing Data of anti-DHX38 antibody

Mouse Anti-DHX38 Monoclonal Antibody – Cat# MBS602008

Western Blot (WB) of anti-DHX38 antibody

Detection of DHX38 by Western Blot. Samples: Whole cell lysate from human HeLa (H, 50ug) , mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells. Predicted molecular weight: 140kD
Mouse Anti-DHX38 Monoclonal Antibody, Western Blot (WB) – Cat# MBS602008

Immunoprecipitation (IP) of anti-DHX38 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DHX38 mAb.
Mouse Anti-DHX38 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS602008

Testing Data of anti-DHX38 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DHX38 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.
Mouse Anti-DHX38 Monoclonal Antibody, Testing Data – Cat# MBS602008

Immunostaining (IS) of anti-DHX38 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DHX38 mAb.
Mouse Anti-DHX38 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS602008

Other Notes

Small volumes of anti-DHX38 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DHX38. It may not necessarily be applicable to this product.

NCBI GI #
119579594
NCBI GeneID
9785
UniProt Primary Accession #
Q92620
UniProt Secondary Accession #
O75212; Q96HN7; D3DWS7
UniProt Related Accession #
Q92620
NCBI Official Full Name
DEAH (Asp-Glu-Ala-His) box polypeptide 38, isoform CRA_a
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 38
NCBI Official Symbol
DHX38
NCBI Official Synonym Symbols
DDX38; PRP16; PRPF16; KIAA0224
UniProt Protein Name
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
UniProt Synonym Protein Names
ATP-dependent RNA helicase DHX38; DEAH box protein 38
UniProt Gene Name
DHX38
UniProt Synonym Gene Names
DDX38; KIAA0224; PRP16
UniProt Entry Name
PRP16_HUMAN
NCBI Protein Information
pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16; OTTHUMP00000174930; DEAH box protein 38; PRP16 homolog of S.cerevisiae; ATP-dependent RNA helicase DHX38; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38; pre-mRNA splicing factor ATP-dependent RNA helicase PRP16

NCBI Summary for DHX38

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process. [provided by RefSeq]
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UniProt Comments for DHX38

DHX38: Probable ATP-binding RNA helicase involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.

Protein type: RNA-binding; Spliceosome; EC 3.6.4.13; RNA processing; RNA splicing; Helicase

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: protein binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DHX38 antibody

 Reference Product  PubMed Publications
 CDC40 antibody  >1 publications with DHX38 and CDC40

Pathways associated with anti-DHX38 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Formation And Maturation Of MRNA Transcript Pathway antibodies  Formation And Maturation Of MRNA Transcript Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Transcription Pathway antibodies  Transcription Pathway Diagram
 Transport Of Mature Transcript To Cytoplasm Pathway antibodies  Transport Of Mature Transcript To Cytoplasm Pathway Diagram

Diseases associated with anti-DHX38 antibody

 Disease Name  Pubmed Publications
 Musculoskeletal Diseases Antibodies  >1 publications with DHX38 and Musculoskeletal Diseases
 Weight Gain Antibodies  >1 publications with DHX38 and Weight Gain
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