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Golgi Marker, 58k Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602047

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to… More details

$860 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Golgi Marker, 58K, Monoclonal Antibody
Full Product Name
Golgi Marker, 58K
Product Synonym Names
Anti -Golgi Marker, 58K
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C140
Host
Mouse
Species Reactivity
Porcine, Bovine, Monkey, Kangaroo, Rat, Hamster, Canine and Human
Form/Format
Supplied as a liquid in 15mM sodium azide.
Purity/Purification
Ascites
Specificity
Recognizes an epitope located on the rat microtubule-binding peripheral Golgi membrane 58K protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Golgi 58K full length from rat liver.
Cellular Localization
Cytoplasmic and Golgi apparatus.
Positive Control
Western Blot (Colometric): Whole rat liver extract.
Indirect Immunofluorescence:(CHO) cells.
Applications Tested/Suitable for anti-Golgi Marker, 58K antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Golgi Marker, 58K antibody
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and as a marker for the Golgi complex in Immunofluorescence.
Dilution: Western Blot: 1:5000-1:10,000; Immunofluorescence (IC): 1:50-1;100

Western Blot (WB) of anti-Golgi Marker, 58K antibody

Western Blot whole cell extract of Golgi membrane fraction from rat liver was separated on SDS-PAGE and blotted with MBS602047. The antibody was developed with Goat Anti-Mouse IgG (Fab Specific), Peroxidase Conjugate and AEC substrate. Lane A: Antibody dilution 1:10,000 Lane B: Negative control (only secondary antibody)
Mouse Anti-Golgi Marker, 58k Monoclonal Antibody – Cat# MBS602047

Immunofluorescence (IF) of anti-Golgi Marker, 58K antibody

Immunofluorescence analysis of HeLa cells were fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS. They were then stained with MBS602047 (1:50) followed by anti-mouse IgG conjugated to Cy3 (1:100). This picture was kindly supplied by Sarah Coleman.
Mouse Anti-Golgi Marker, 58k Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS602047

Immunofluorescence (IF) of anti-Golgi Marker, 58K antibody

Immunofluorescence of Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody MBS602047
Mouse Anti-Golgi Marker, 58k Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS602047

Other Notes

Small volumes of anti-Golgi Marker, 58K antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Golgi Marker, 58K purchase

MBS602047 is an antibody targeting Golgi Marker, 58K. Reactive in Porcine, Bovine, Monkey, Kangaroo, Rat, Hamster, Canine and Human. Host: Mouse.
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