Mouse Anti-CD101 Monoclonal Antibody – Cat# MBS602060

CD101 Antibody – Mouse Monoclonal

Name: CD101 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS602060
Price: 820 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602060

CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. More details

$820 / 100 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD101, Monoclonal Antibody

Full Product Name

CD101 (PE)

Product Gene Name

anti-Cd101 antibody

Product Synonym Names

Anti -CD101 (PE)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

4j29

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml ddH2O.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (1).

Preparation and Storage

Lyophilized powder may be stored at 4 degree C. Reconstitute with ddH2O. Aliquot and store at 4 degree C. Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Molecular Weight

114,205 Da

Chromosome Location

chromosome: 3; Location: 3 F2.2; 3

3D Structure

ModBase 3D Structure for A8E0Y8

Immunogen

Human thymic clone B12

Recommended Negative Controls

I1904-79D: IgG1 Murine Negative Control (PE)

Product Categories/Family for anti-Cd101 antibody

Antibodies; Abs to CD Markers

Applications Tested/Suitable for anti-Cd101 antibody

Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Application Notes for anti-Cd101 antibody

Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation.
Dilution: Flow Cytometry: Neat -1:5. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: frozen samples

Other Notes

Small volumes of anti-Cd101 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cd101 purchase

MBS602060 is an antibody targeting Cd101. Also known as CD101 (PE). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Cd101. It may not necessarily be applicable to this product.

NCBI GI #

157390470

NCBI GeneID

630146

UniProt Primary Accession #

A8E0Y8

UniProt Secondary Accession #

A8E0Y9

UniProt Related Accession #

A8E0Y8

NCBI Official Full Name

CD101 protein

NCBI Official Synonym Full Names

CD101 antigen

NCBI Official Symbol

Cd101

NCBI Official Synonym Symbols

Gm734; Igsf2; Gm1016; Cd101

UniProt Protein Name

Immunoglobulin superfamily member 2

UniProt Synonym Protein Names

Glu-Trp-Ile EWI motif-containing protein 101

Protein Family

Immunoglobulin superfamily

UniProt Gene Name

Cd101

UniProt Synonym Gene Names

Igsf2

UniProt Entry Name

IGSF2_MOUSE

NCBI Protein Information

immunoglobulin superfamily member 2; EWI-101; leukocyte surface protein; immunoglobulin superfamily, member 2; EWI immunoglobulin subfamily member EWI-101; glu-Trp-Ile EWI motif-containing protein 101

UniProt Comments for Cd101

IGSF2: Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3- induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: membrane; integral to membrane

Biological Process: positive regulation of myeloid leukocyte differentiation

Research Articles on Cd101

1. of the eight genes found in the Idd10 region, the CD101 gene was selected as a candidate for Idd10 based on its function in humans and its polymorphism in the NOD strain of mice
2. Cd101, but not Fcgr1, is responsible for the Idd10 effect

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-Cd101 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>2 publications with Cd101 and Skin