Mouse Anti-Ficolin 2 Monoclonal Antibody – Cat# MBS602111

Ficolin 2 Antibody – Mouse Monoclonal

Name: Ficolin 2 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS602111
Price: 970 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602111

Ficolin-2 is a lectin that contains fibrinogen-like and collagen-like domains. It circulates as a tetramer of disulfide-linked homotrimers. Ficolin-2 associates with MASP proteins to activate complement. More details

$970 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ficolin 2, Monoclonal Antibody

Full Product Name

Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)

Product Synonym Names

Anti -Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

8H139

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human Ficolin-2. Does not crossreact with rhFicolin-3.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

NS0-derived rhFicolin-2

Hybridoma

Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Ficolin-2 (rhFicolin-2).

Product Categories/Family for anti-Ficolin 2 antibody

Antibodies; Abs to Lectins

Applications Tested/Suitable for anti-Ficolin 2 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-Ficolin 2 antibody

Western Blot, Direct ELISA
Dilution: Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human Ficolin-2. Using a colorimetric detection system, the detection limit for rhFicolin-2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human Ficolin-2. The detection limit for rhFicolin-2 is approximately 1 ng/well.

Other Notes

Small volumes of anti-Ficolin 2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ficolin 2 purchase

MBS602111 is an antibody targeting Ficolin 2. Also known as Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Ficolin 2. It may not necessarily be applicable to this product.

NCBI GI #

1911530

NCBI Accession #

AAB50706.1

NCBI Official Full Name

ficolin

Protein Family

Ficolin

UniProt Comments for Ficolin 2

FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extrinsic to external side of plasma membrane; collagen; extracellular region

Molecular Function: protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; sialic acid binding

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.