Verotoxin I-B Subunit Antibody – Mouse Monoclonal

Shiga-like toxins, also called verotoxins, inhibit protein synthesis in eukaryotic cells. These toxins play a role in hemorrhagic colitis and hemolytic uremic syndrome. They damage endothelial cells in both kidney and brain, causing renal failure and neurological complications. The protein structure consists of two domains: the A polypeptide confers the activity, and the B polypeptide pentamer confers the receptor-specific binding. The A polypeptide has an N-glycosidase activity that cleaves an adenine from the 28S rRNA of the 60S cytoplasmic ribosome. This activity renders the 28S rRNA unable to interact with the elongation factors EF-1 and EF-2, thus inhibiting protein synthesis. The B polypeptide forms a pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3). Shiga-like toxins then enter the cells by receptor-mediated endocytosis. The effects of shiga-like toxin 1 and shiga-like toxin 2 have been shown to vary with cell type because Gb3 receptors are present in certain tissues, particularly sensory neurons and renal cells. In those cell types, the receptor concentration and binding is modulated by factors such as tumor necrosis factor (TNF), interleukins, and fatty acid content of the membranes. Shiga-like toxin 1 and shiga-like toxin 2 differ in their effects on various tissues. They have both been shown to induce apoptosis in several different cell types. These toxins have many interesting effects at the cellular level. Once the shiga-like toxins have been endocytosed, they are retrogradely transported through the golgi apparatus to the rough endoplasmic reticulum where they effectively target the ribosomes. In addition to inhibiting protein synthesis, shiga- like toxins induce cytokines such as interleukin-1, interleukin-6, and interleukin-8. They have also been shown to induce expression of tumor necrosis factor (TNF), induce F-actin depolymerization, and activate a src kinase. Shiga-like toxin 1 and 2 are from E. coli. Shiga-like toxins are very toxic and should be handled with extreme caution.

Small volumes of anti-Verotoxin I-B subunit antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS602119 is an antibody targeting Verotoxin I-B subunit. Also known as Verotoxin I-B subunit (Shiga-like, SLT-1b). Host: Mouse.