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XRCC1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602401

X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half.. In testis XRCC1 is… More details

$750 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
XRCC1, Monoclonal Antibody
Full Product Name
XRCC1 (X-Ray Repair Cross Complementing Protein1)
Product Gene Name
Product Synonym Names
Anti -XRCC1 (X-Ray Repair Cross Complementing Protein1)
Immunogen Sequence
Not determined
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3F399
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See X9501X
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes XRCC1 (X-Ray Repair Cross Complementing Protein1). Species Crossreactivity: human.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
85kD
Chromosome Location
Chromosome: 19; NC_000019.9 (44047464..44079730, complement). Location: 19q13.2
OMIM
194360
3D Structure
ModBase 3D Structure for P18887
Immunogen
Recombinant human XRCC1 protein.
Cellular Localization
Nuclear
Positive Control
Ls174T, or HeLa cells. Normal testis.
Applications Tested/Suitable for anti-XRCC1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-XRCC1 antibody
Dilution: Immunoprecipitation (Denatured verified) (Use Protein A) (Ab 2ug/mg protein lysate)
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Immunoprecipitation (IP) of anti-XRCC1 antibody

Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate) |Western Blotting (Ab 1-2ug/ml for 2hrs at RT) =
Mouse Anti-XRCC1 Monoclonal Antibody – Cat# MBS602401

Immunohistochemistry (IHC) of anti-XRCC1 antibody

Formalin-fixed, paraffin-embedded human testis stained with XRCC1 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.
Mouse Anti-XRCC1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS602401

Other Notes

Small volumes of anti-XRCC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for XRCC1 purchase

MBS602401 is an antibody targeting XRCC1. Also known as XRCC1 (X-Ray Repair Cross Complementing Protein1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for XRCC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
XRCC1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol
XRCC1
NCBI Official Synonym Symbols
RCC
UniProt Protein Name
DNA repair protein XRCC1
UniProt Synonym Protein Names
X-ray repair cross-complementing protein 1
Protein Family
UniProt Gene Name
XRCC1
UniProt Entry Name
XRCC1_HUMAN
NCBI Protein Information
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster

NCBI Summary for XRCC1

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]
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UniProt Comments for XRCC1

XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; damaged DNA binding

Biological Process: single strand break repair; base-excision repair; DNA repair
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-XRCC1 antibody

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