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Ser/Thr, Pro (MPM2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602417

Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho aa-containing epitope (peptides containing LTPLK and FTPLQ domains) present on… More details

$1055 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MPM2, Ser/Thr, Pro, Monoclonal Antibody
Full Product Name
MPM2, Ser/Thr, Pro, phosphorylated (Mitotic Protein Monoclonal#2)
Product Synonym Names
Anti -MPM2, Ser/Thr, Pro, phosphorylated (Mitotic Protein Monoclonal#2)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.T.181
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human MPM2. Reacts with phosphorylated serines or threonines when followed by a proline. Recognizes a phosphorylated epitope (S/T)P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM2 varies from species to species and with the cell type. Species Crossreactivity: animals and plants.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mitotic human HeLa cell cytosolic lysate.
Positive Control
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde.

Product Categories/Family for anti-MPM2, Ser/Thr, Pro antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-MPM2, Ser/Thr, Pro antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-MPM2, Ser/Thr, Pro antibody
Suitable for use in Western Blot, Immunocytochemistry, ELISA, Immunohistochemistry, Immunoprecipitation and Flow Cytometry.
Dilution: Western Blot: 0.5-2ug/ml detects mitosis-associated proteins in colcemid treated (0.05ug/ml) human HeLa carcinoma cells. Colcemid-treated HeLa cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with M4685. Proteins were visualized using an IgG (HRP) goat anti-mouse antibody and ECL.
Immunocytochemistry: 5-15ug/ml shows positive staining of mitotic cells.
ELISA: Used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassay (DELPHIA). Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with a europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin).
Immunoprecipitation: Reported by an independent laboratory.

Western Blot (WB) of anti-MPM2, Ser/Thr, Pro antibody

Western Blot analysis using MBS602417 (2ug/ml):|Lane 1: Non-treated HeLa cell lysates |Lane 2: colcemid-treated HeLa cell lysates
Mouse Anti-MPM2 Monoclonal Antibody – Cat# MBS602417

Immunostaining (IS) of anti-MPM2, Ser/Thr, Pro antibody

MPM-2 immunostaining (green) of phospho-proteins in metaphase cells using MBS602417. The spindle, centrosome and centromeric regions are stained. DNA is counterstained with propidium iodide (red).
Mouse Anti-MPM2 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS602417

Flow Cytometry (FC/FACS) of anti-MPM2, Ser/Thr, Pro antibody

Flow Cytometry analysis of HeLa cells were treated with colcemid (green), or untreated (grey) using MBS602417.
Mouse Anti-MPM2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS602417

Testing Data of anti-MPM2, Ser/Thr, Pro antibody

Mouse Anti-MPM2 Monoclonal Antibody, Testing Data – Cat# MBS602417

Other Notes

Small volumes of anti-MPM2, Ser/Thr, Pro antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MPM2, Ser/Thr, Pro purchase

MBS602417 is an antibody targeting MPM2, Ser/Thr, Pro. Also known as MPM2, Ser/Thr, Pro, phosphorylated (Mitotic Protein Monoclonal#2). Reactive in Human. Host: Mouse.
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