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Heat Shock Protein 70, Universal (HYOU1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602471

MBS602471 is an antibody targeting Hyou1. Also known as Heat Shock Protein 70, Universal (HSP70). Reactive in Avian, Drosophila, Frog, Human, Mouse. Host: Mouse. More details

$1195 / 0.25 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Heat Shock Protein 70, Universal (Hyou1), Monoclonal Antibody
Full Product Name
Heat Shock Protein 70, Universal (HSP70)
Product Gene Name
Product Synonym Names
Anti -Heat Shock Protein 70, Universal (HSP70)
Clonality
Monoclonal
Isotype
IgG
Clone Number
6D890
Host
Mouse
Species Reactivity
Avian, Drosophila, Frog, Human, Mouse
Form/Format
Supplies as a liquid in PBS
Purity/Purification
Ascites
Ascites
Specificity
Recognizes several members of the HSP70 gene family, including HSP70, HSC70, GRP78, and following heat shock, HSP72 in a broad range of species, including human, mouse, avian, frog, fish, drosophila, and wheat HSP70. Detects bands from ~70 to ~78kDa by Western blot, representing different members of the HSP70 family. 2-D-gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
111,181 Da
Chromosome Location
Chromosome: 9; NC_000075.6 (44379490..44392369). Location: 9; 9 B
3D Structure
ModBase 3D Structure for Q9JKR6
Immunogen
Recombinant HSP70 (heat shock protein 70).
Applications Tested/Suitable for anti-Hyou1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-Hyou1 antibody
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 1:1000.
Immunocytochemistry: 1:100.

Other Notes

Small volumes of anti-Hyou1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hyou1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hypoxia up-regulated protein 1
NCBI Official Synonym Full Names
hypoxia up-regulated 1
NCBI Official Symbol
Hyou1
NCBI Official Synonym Symbols
Cab140; Grp170; Orp150; CBP-140; AI415631
UniProt Protein Name
Hypoxia up-regulated protein 1
UniProt Synonym Protein Names
140 kDa Ca(2+)-binding protein
UniProt Gene Name
Hyou1
UniProt Synonym Gene Names
Grp170; GRP-170; CBP-140
UniProt Entry Name
HYOU1_MOUSE
NCBI Protein Information
hypoxia up-regulated protein 1; GRP-170; calcium binding protein 140; 140 kDa Ca(2+)-binding protein; 150-kDa oxygen regulated protein; calcium binding protein, 140 kDa; 170 kDa glucose regulated protein GRP170

UniProt Comments for Hyou1

HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein; Chaperone

Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum

Molecular Function: nucleotide binding; ATP binding

Biological Process: response to hypoxia; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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