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Brix Domain Containing 1 (RPF2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602522

MBS602522 is an antibody targeting RPF2. Also known as Brix Domain Containing 1 (BXDC1, bA397G5.4, FLJ21087, RPF2). Reactive in Human. Host: Mouse. More details

$850 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Brix Domain Containing 1 (RPF2), Monoclonal Antibody
Full Product Name
Brix Domain Containing 1 (BXDC1, bA397G5.4, FLJ21087, RPF2)
Product Gene Name
Product Synonym Names
Anti -Brix Domain Containing 1 (BXDC1, bA397G5.4, FLJ21087, RPF2)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C38
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Brix Domain Containing 1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
35,583 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (111303291..111346794). Location: 6q21
3D Structure
ModBase 3D Structure for Q9H7B2
Immunogen
Partial sequence of recombinant full-length protein to protein corresponding to amino acids located near the C-terminal region of human Brix Domain Containing 1
Applications Tested/Suitable for anti-RPF2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
Application Notes for anti-RPF2 antibody
Suitable for use in Immunocytochemistry, Dot Blot, and Western Blot.

Western Blot (WB) of anti-RPF2 antibody

Western Blot analysis using immunogen as sample.
Mouse Anti-RPF2 Monoclonal Antibody – Cat# MBS602522

Western Blot (WB) of anti-RPF2 antibody

Detection of human BXDC1 by Western blot. Samples: Whole cell lysate (25ug) from HeLa cells. Predicted molecular weight: 35 kD
Mouse Anti-RPF2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS602522

Immunostaining (IS) of anti-RPF2 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with B758-10. Nucleus was stained with Hoechst (blue fluorescence).
Mouse Anti-RPF2 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS602522

Other Notes

Small volumes of anti-RPF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Brix domain containing 1
NCBI Official Synonym Full Names
ribosome production factor 2 homolog (S. cerevisiae)
NCBI Official Symbol
RPF2
NCBI Official Synonym Symbols
BXDC1; FLJ21087; bA397G5.4
UniProt Protein Name
Ribosome production factor 2 homolog
UniProt Synonym Protein Names
Brix domain-containing protein 1; Ribosome biogenesis protein RPF2 homolog
UniProt Gene Name
RPF2
UniProt Synonym Gene Names
BXDC1
UniProt Entry Name
RPF2_HUMAN
NCBI Protein Information
ribosome production factor 2 homolog; homolog of Rpf2; OTTHUMP00000017009; OTTHUMP00000017010; OTTHUMP00000017011; brix domain containing 1; brix domain-containing protein 1; ribosomal processing factor 2 homolog; ribosome biogenesis protein RPF2 homolog

UniProt Comments for RPF2

BXDC1: Belongs to the RPF2 family.

Protein type: Nucleolus; Unknown function

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleolus; nucleus

Molecular Function: rRNA binding

Biological Process: ribosomal large subunit assembly and maintenance; maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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