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Nuclei and Chromosomes, Histone Specific Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602552

MBS602552 is an antibody targeting Nuclei and Chromosomes, Histone specific. Reactive in Human. Host: Mouse. More details

$1045 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nuclei and Chromosomes, Histone specific, Monoclonal Antibody
Full Product Name
Nuclei and Chromosomes, Histone specific
Product Synonym Names
Anti -Nuclei and Chromosomes, Histone specific
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2Q2232
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Histone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts preferentially with Histone H1 by ELISA and immunoblot. Produces a speckled nuclear staining pattern in all tissues studies. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Positive Control
Human tonsil
ELISA (EL/EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Suitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence and Immunohistochemistry.
Dilution: Immunocytochemistry (Indirect immunofluorescence): 1:20-1:30, 30-100ul per slide/well using paraformaldehyde/acetone fixed cell preparation.
Immunohistochemistry: Formalin fixed, paraffin embedded tissue sections or paraformaldehyde/acetone fixed frozen tissue sections.
Optimal dilution determined by the researcher.

Other Notes

Small volumes of anti-Nuclei and Chromosomes, Histone specific antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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