Mouse Anti-CENPF Monoclonal Antibody – Cat# MBS602557

CENP-F (CENPF) Antibody – Mouse Monoclonal

Name: CENPF Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS602557
Price: 850 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602557

MBS602557 is an antibody targeting CENPF. Also known as CENP-F (Centromere protein F, CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K… More details

$850 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CENP-F (CENPF), Monoclonal Antibody

Full Product Name

CENP-F (Centromere protein F, CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein)

Product Gene Name

anti-CENPF antibody

Product Synonym Names

Anti -CENP-F (Centromere protein F, CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

8C70

Host

Mouse

Form/Format

Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human CENP-F.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

367,764 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (214776532..214837914). Location: 1q32-q41

OMIM

600236

3D Structure

ModBase 3D Structure for P49454

Immunogen

Partial sequence of recombinant full-length protein to human CENP-F

Product Categories/Family for anti-CENPF antibody

Antibodies; Abs to Centromeres

Applications Tested/Suitable for anti-CENPF antibody

Western Blot (WB)

Application Notes for anti-CENPF antibody

Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000

Other Notes

Small volumes of anti-CENPF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CENPF. It may not necessarily be applicable to this product.

NCBI GI #

55770834

NCBI GeneID

1063

NCBI Accession #

NP_057427.3

UniProt Primary Accession #

P49454

UniProt Secondary Accession #

Q13171; Q13246; Q5VVM7

UniProt Related Accession #

P49454; Q9UHT7

GenBank Nucleotide #

NM_016343.3

NCBI Official Full Name

centromere protein F

NCBI Official Synonym Full Names

centromere protein F, 350/400kDa (mitosin)

NCBI Official Symbol

CENPF

NCBI Official Synonym Symbols

CENF; hcp-1; PRO1779

UniProt Protein Name

Centromere protein F

UniProt Synonym Protein Names

AH antigen; Kinetochore protein CENPF; Mitosin

Protein Family

Centromere protein

UniProt Gene Name

CENPF

UniProt Entry Name

CENPF_HUMAN

NCBI Protein Information

centromere protein F; mitosin; AH antigen; OTTHUMP00000035028; kinetochore protein CENPF; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin)

NCBI Summary for CENPF

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]

UniProt Comments for CENPF

CENPF: a nuclear matrix protein that assembles onto kinetochores at late G2. Is hyperphosphorylated during mitosis and rapidly degraded after mitosis. Involved in chromosome segregation during mitosis. Interacts with retinoblastoma protein (RB), CENP-E and BUBR1. Two alternatively spliced human isoforms have been described.

Protein type: Transcription, coactivator/corepressor; Nuclear envelope; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: spindle pole; nuclear matrix; pronucleus; nuclear envelope; cytosol; nucleoplasm; kinetochore; perinuclear region of cytoplasm; cytoplasm; outer kinetochore of condensed chromosome; spindle; midbody; nucleus; chromatin; chromosome, pericentric region

Molecular Function: protein C-terminus binding; dynein binding; protein binding; protein homodimerization activity; chromatin binding; transcription factor binding

Biological Process: response to drug; mitosis; muscle development; kinetochore assembly; regulation of cell cycle; chromosome segregation; cell proliferation; protein transport; cell division; mitotic cell cycle spindle assembly checkpoint; regulation of G2/M transition of mitotic cell cycle; metaphase plate congression; mitotic cell cycle; G2/M transition of mitotic cell cycle; cell differentiation; negative regulation of transcription, DNA-dependent; regulation of striated muscle development

Disease: Ciliary Dyskinesia, Primary, 31

Research Articles on CENPF

1. Data identified Cenp-F as a potential new molecular target for NBPs in tumour cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CENPF antibody

Reference Product	PubMed Publications
CENPE antibody	>3 publications with CENPF and CENPE

Pathways associated with anti-CENPF antibody

Products by Pathway	Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
FOXM1 Transcription Factor Network Pathway antibodies	FOXM1 Transcription Factor Network Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic M-M/G1 Phases Pathway antibodies	Mitotic M-M/G1 Phases Pathway Diagram
Mitotic Prometaphase Pathway antibodies	Mitotic Prometaphase Pathway Diagram

Diseases associated with anti-CENPF antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>9 publications with CENPF and Neoplasms
Carcinoma Antibodies	>3 publications with CENPF and Carcinoma
Breast Neoplasms Antibodies	>3 publications with CENPF and Breast Neoplasms
Carcinoma, Squamous Cell Antibodies	>2 publications with CENPF and Carcinoma, Squamous Cell
Glioblastoma Antibodies	>2 publications with CENPF and Glioblastoma
Head and Neck Neoplasms Antibodies	>2 publications with CENPF and Head and Neck Neoplasms
Esophageal Neoplasms Antibodies	>2 publications with CENPF and Esophageal Neoplasms
Neuroectodermal Tumors Antibodies	>2 publications with CENPF and Neuroectodermal Tumors
Monosomy Antibodies	>1 publications with CENPF and Monosomy
Neoplasm Metastasis Antibodies	>1 publications with CENPF and Neoplasm Metastasis

Organs/Tissues associated with anti-CENPF antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>2 publications with CENPF and Kidney
Lymph Node Antibodies	>2 publications with CENPF and Lymph Node
Muscle Antibodies	>1 publications with CENPF and Muscle
Brain Antibodies	>1 publications with CENPF and Brain