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RNA Polymerase II, TATA Box Binding Protein (TAF9B) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602929

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly,… More details

$850 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TAF9B RNA Polymerase II, TATA Box Binding Protein, Monoclonal Antibody
Full Product Name
TAF9B RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 31kD (TAF9B, DN7, DN-7, TAFII31L, TFIID-31)
Product Gene Name
anti-TAF9B antibody
Product Synonym Names
Anti -TAF9B RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 31kD (TAF9B, DN7, DN-7, TAFII31L, TFIID-31)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8C317
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human TAF9B RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 31kD. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
27,622 Da
Chromosome Location
Chromosome: X; NC_000023.10 (77385245..77395179, complement). Location: Xq13.1-q21.1
OMIM
300754
3D Structure
ModBase 3D Structure for Q9HBM6
Immunogen
Partial sequence of recombinant full-length protein to human TAF9B RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 31kD

Product Categories/Family for anti-TAF9B antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-TAF9B antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-TAF9B antibody
Suitable for use in Western Blot and Immunocytochemistry.

Testing Data of anti-TAF9B antibody

Mouse Anti-TAF9B Monoclonal Antibody – Cat# MBS602929

Western Blot (WB) of anti-TAF9B antibody

Detection of TAF9L by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 27 kD
Mouse Anti-TAF9B Monoclonal Antibody, Western Blot (WB) – Cat# MBS602929

Immunostaining (IS) of anti-TAF9B antibody

Immunostaining analysis in HeLa cells. HeLa cells were|fixed with 4% paraformaldehyde and permeabilized with|0.1% Triton X-100 in PBS. The cells were immunostained|with MBS602929
Mouse Anti-TAF9B Monoclonal Antibody, Immunostaining (IS) – Cat# MBS602929

Other Notes

Small volumes of anti-TAF9B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TAF9B purchase

MBS602929 is an antibody targeting TAF9B. Also known as TAF9B RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 31kD (TAF9B, DN7, DN-7, TAFII31L, TFIID-31). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TAF9B. It may not necessarily be applicable to this product.

NCBI GI #
119619007
NCBI GeneID
51616
UniProt Primary Accession #
Q9HBM6
UniProt Secondary Accession #
Q9Y2S3; B2RUZ9
UniProt Related Accession #
Q9HBM6
NCBI Official Full Name
TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa, isoform CRA_b
NCBI Official Synonym Full Names
TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
NCBI Official Symbol
TAF9B
NCBI Official Synonym Symbols
DN7; DN-7; TAF9L; TAFII31L; TFIID-31
UniProt Protein Name
Transcription initiation factor TFIID subunit 9B
UniProt Synonym Protein Names
Neuronal cell death-related protein 7; DN-7; Transcription initiation factor TFIID subunit 9-like; Transcription-associated factor TAFII31L
Protein Family
Transcription initiation factor
UniProt Gene Name
TAF9B
UniProt Synonym Gene Names
TAF9L
UniProt Entry Name
TAF9B_HUMAN
NCBI Protein Information
transcription initiation factor TFIID subunit 9B; OTTHUMP00000023594; TBP-associated factor 9L; neuronal cell death-related protein 7; transcription associated factor TAFII31L; transcription-associated factor TAFII31L; transcription initiation factor IID, 31kD subunit; TAF9-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa

UniProt Comments for TAF9B

TAF9B: Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. Belongs to the TAF9 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq13.1-q21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; protein heterodimerization activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; protein stabilization; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of apoptosis
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Research Articles on TAF9B

1. TAF9b (TAF9L) was identified as a subunit of TFIID.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TAF9B antibody

 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
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