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Akt, Dephosphorylated Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS602934

The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kiinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene.… More details

$865 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Akt, dephosphorylated, Monoclonal Antibody
Full Product Name
Akt, dephosphorylated (Ser473) (Rac PKa, PKBa)
Product Synonym Names
Anti -Akt, dephosphorylated (Ser473) (Rac PKa, PKBa)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6D108
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide PEG and sucrose. Reconstitute with 1ml sterile, ddH2O. (15 min, RT).
Purity/Purification
Purified
Purified.
Specificity
Recognizes human Akt dephosphorylated at Ser473. Does not cross-react with Akt phosphorylated at Ser473. Species Crossreactivity: mouse.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Immunogen
Synthetic peptide corresponding to C-terminal (QFSYSA) human Akt (KLH).
Positive Control
A1124-02D1 Cell lysate from untreated HepG2 cells.
Applications Tested/Suitable for anti-Akt, dephosphorylated antibody
ELISA (EL/EIA), Western Blot (WB)
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.05ug/ml
Western Blot: 1ug/ml (AP)
Western Blot: 0.5 ug/ml (HRP/ECL)

Other Notes

Small volumes of anti-Akt, dephosphorylated antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Akt, dephosphorylated purchase

MBS602934 is an antibody targeting Akt, dephosphorylated. Also known as Akt, dephosphorylated (Ser473) (Rac PKa, PKBa). Reactive in Human, Mouse. Host: Mouse.
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