Mouse Anti-CHD3 Monoclonal Antibody – Cat# MBS603012

CHD3 Antibody – Mouse Monoclonal

Name: CHD3 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS603012
Price: 685 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603012

MBS603012 is an antibody targeting CHD3. Also known as CHD3 (Chromodomain Helicase DNA Binding Protein 3, Mi-2a, Mi2-alpha, ZFH). Host: Mouse. More details

$685 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CHD3, Monoclonal Antibody

Full Product Name

CHD3 (Chromodomain Helicase DNA Binding Protein 3, Mi-2a, Mi2-alpha, ZFH)

Product Gene Name

anti-CHD3 antibody

Product Synonym Names

Anti -CHD3 (Chromodomain Helicase DNA Binding Protein 3, Mi-2a, Mi2-alpha, ZFH)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

8C72

Host

Mouse

Form/Format

Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human CHD3.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

226,592 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (7788123..7816075). Location: 17p13.1

OMIM

602120

3D Structure

ModBase 3D Structure for Q12873

Immunogen

Partial sequence of recombinant full-length protein to human CHD3

Product Categories/Family for anti-CHD3 antibody

Antibodies; Abs to Binding Proteins

Applications Tested/Suitable for anti-CHD3 antibody

Western Blot (WB)

Application Notes for anti-CHD3 antibody

Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000

Other Notes

Small volumes of anti-CHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHD3. It may not necessarily be applicable to this product.

NCBI GI #

158420731

NCBI GeneID

1107

NCBI Accession #

NP_001005271.2

UniProt Primary Accession #

Q12873

UniProt Secondary Accession #

Q9Y4I0; D3DTQ9

UniProt Related Accession #

Q12873; Q2TAZ1

GenBank Nucleotide #

NM_001005271.2

NCBI Official Full Name

chromodomain-helicase-DNA-binding protein 3 isoform 3

NCBI Official Synonym Full Names

chromodomain helicase DNA binding protein 3

NCBI Official Symbol

CHD3

NCBI Official Synonym Symbols

ZFH; Mi-2a; Mi2-ALPHA

UniProt Protein Name

Chromodomain-helicase-DNA-binding protein 3

UniProt Synonym Protein Names

ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase

Protein Family

Chromodomain-helicase-DNA-binding protein

UniProt Gene Name

CHD3

UniProt Entry Name

CHD3_HUMAN

NCBI Protein Information

chromodomain-helicase-DNA-binding protein 3; hZFH; CHD-3; OTTHUMP00000197335; OTTHUMP00000197336; OTTHUMP00000197337; zinc finger helicase; ATP-dependent helicase CHD3; mi-2 autoantigen 240 kDa protein; zinc-finger helicase (Snf2-like)

NCBI Summary for CHD3

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

UniProt Comments for CHD3

CHD-3 iso3: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity. Widely expressed. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; centrosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; zinc ion binding; helicase activity; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; centrosome organization and biogenesis; chromatin modification; spindle organization and biogenesis; DNA duplex unwinding

Research Articles on CHD3

1. UV radiation regulates Mi-2 through protein translation and stability

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHD3 antibody

Reference Product	PubMed Publications
TRIM28 antibody	>1 publications with CHD3 and TRIM28
SUMO1 antibody	>1 publications with CHD3 and SUMO1

Pathways associated with anti-CHD3 antibody

Products by Pathway	Pathway Diagram
Signaling Events Mediated By HDAC Class I Pathway antibodies	Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with anti-CHD3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>4 publications with CHD3 and Neoplasms
Cardiovascular Diseases Antibodies	>1 publications with CHD3 and Cardiovascular Diseases

Organs/Tissues associated with anti-CHD3 antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>3 publications with CHD3 and Kidney
Nerve Antibodies	>3 publications with CHD3 and Nerve
Brain Antibodies	>2 publications with CHD3 and Brain
Bone Antibodies	>1 publications with CHD3 and Bone
Adrenal Gland Antibodies	>1 publications with CHD3 and Adrenal Gland
Skin Antibodies	>1 publications with CHD3 and Skin
Pancreas Antibodies	>1 publications with CHD3 and Pancreas
Embryonic Tissue Antibodies	>1 publications with CHD3 and Embryonic Tissue
Vascular Antibodies	>1 publications with CHD3 and Vascular
Muscle Antibodies	>1 publications with CHD3 and Muscle